Saliva biomonitoring using LPME-GC/MS method to assess dentistry exposure to plasticizers

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dc.creatorJosé Messias Gomes
dc.creatorTatiana Fernandes Araujo Almeida
dc.creatorTarcília Aparecida da Silva
dc.creatorZenilda de Lourdes Cardeal
dc.creatorHelvécio Costa Menezes
dc.date.accessioned2023-01-11T19:05:37Z
dc.date.accessioned2025-09-08T22:57:53Z
dc.date.available2023-01-11T19:05:37Z
dc.date.issued2020
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1007/s00216-020-02908-x
dc.identifier.issn1618-2642
dc.identifier.urihttps://hdl.handle.net/1843/48867
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAnalytical and Bioanalytical Chemistry
dc.rightsAcesso Restrito
dc.subjectQuímica analítica
dc.subjectExtração (Química)
dc.subjectCromatografia de gás
dc.subjectEspectrometria de massa
dc.subjectSaliva
dc.subjectPreparação de amostra (Química)
dc.subjectTratamento dentário
dc.subject.otherBiomonitoramento de saliva
dc.subject.otherMétodo LPME-GC / MS
dc.subject.otherExposição odontológica a plastificantes
dc.subject.otherEfeitos do bisfenol A
dc.subject.otherMicroextração em fase líquida
dc.subject.otherPatologia Bucal
dc.subject.otherGas chromatography
dc.subject.otherLiquid phase microextraction
dc.subject.otherSaliva
dc.subject.otherPhthalate
dc.subject.otherBisphenol A
dc.subject.otherBiomonitoring
dc.titleSaliva biomonitoring using LPME-GC/MS method to assess dentistry exposure to plasticizers
dc.typeArtigo de periódico
local.citation.epage7810
local.citation.issue28
local.citation.spage7799
local.citation.volume412
local.description.resumoBisphenol A and phthalates are endocrine disruptors widely used as chemical additives mainly in plastic products, including materials for dentistry procedures. Besides, many plasticizers have been associated with important diseases requiring performed methods for their quantification. In the present study, an alternative method for the determination of bisphenol A (BPA) and phthalate metabolites in saliva was developed and validated using hollow fiber liquid phase microextraction (HF-LPME) for sample preparation and gas chromatography coupled to ion trap mass spectrometry (GC/MS) for analysis. A mixture of octanol and ethyl octanoate (1:1 v/v) was used as an acceptor phase in hollow fiber to extract the analytes from saliva samples. A Doehlert design was performed to optimize the variable sample agitation and extraction time. The HF-LPME-GC/MS method developed for saliva analysis showed good selectivity, linearity (R2 > 0.900), and precision (CV = 0.86–18.68%). Limits of detection and quantification ranged from 0.03 to 0.53 μg L−¹ and 0.09 to 1.78 μg L−¹, respectively. A high concentration of BPA in the oral cavity and oropharyngeal space is a warning of the possible association with the main cancer of the mouth. The method developed and validated was applied to patients with oral squamous cell carcinoma (study group, n = 16) and patients who did not present any oral lesion (control group, n = 16). A principal component analysis was performed and showed a tendency for the association between oral squamous cell carcinoma (OSCC) and plasticizers.
local.identifier.orcidhttps://orcid.org/0000-0003-1383-6299
local.identifier.orcidhttps://orcid.org/0000-0002-5759-612X
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.departmentFAO - FACULDADE DE ODONTOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s00216-020-02908-x

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