KR12 peptide associated with cyclodextrin: antimicrobial and antitumor activities

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dc.creatorKarina Imaculada Rosa Teixeira
dc.creatorMaría Esperanza Cortés Segura
dc.creatorRobson Augusto Souza dos Santos
dc.creatorFabio de Oliveira
dc.creatorRubén Dario Sinisterra Millán
dc.date.accessioned2023-12-04T13:04:47Z
dc.date.accessioned2025-09-09T00:08:05Z
dc.date.available2023-12-04T13:04:47Z
dc.date.issued2016-12
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)
dc.identifier.doihttps://doi.org/10.1116/1.4968880
dc.identifier.issn1559-4106
dc.identifier.urihttps://hdl.handle.net/1843/61669
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBiointerphases
dc.rightsAcesso Restrito
dc.subjectQuímica supramolecular
dc.subjectCiclodextrinas
dc.subjectAgentes antineoplásicos
dc.subjectAgentes antiinfecciosos
dc.subjectColóides
dc.subjectPeptídios
dc.subjectCélulas epiteliais
dc.subjectHemólise e hemolisinas
dc.subjectPotencial zeta
dc.subjectEfeito citotóxico
dc.subject.otherColloids
dc.subject.otherChemical compounds
dc.subject.otherAntimicrobials
dc.subject.otherMicrobioassays
dc.subject.otherBacteria
dc.subject.otherCytotoxicity
dc.subject.otherPeptides
dc.subject.otherEpithelial cells
dc.subject.otherHemolysis
dc.subject.otherCell lines
dc.titleKR12 peptide associated with cyclodextrin: antimicrobial and antitumor activities
dc.typeArtigo de periódico
local.citation.epage04B307-7
local.citation.issue4
local.citation.spage04B307-1
local.citation.volume11
local.description.resumoThe aim of this study was to determine the physical properties and antimicrobial and antiproliferative effects of the KR12 peptide complexed with 2-hydroxypropyl-β-cyclodextrin (Hp-βCd) in vitro. The KR12:Hp-βCd composition was evaluated for particle size and its zeta (ζ)-potential in the presence and absence of cells. Antimicrobial activity against Streptococcus mutans, Actinobacillus actinomycetemcomitans, and Porphyromonas gingivalis for the peptide alone or associated was evaluated by minimal inhibitory concentration. The cytotoxicity of the peptide and composition toward fibroblasts, Caco-2 cells, and A431 cells was determined using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; thiazolyl blue assay and hemolysis assay. Membrane integrity was analyzed by the lactate dehydrogenase assay. KR12:Hp-βCd decreased the peptide concentration required for the antimicrobial effect. Moreover, this composition was able to modify cell surface parameters, such as ζ-potential, and alter the degree of hemolysis induced by KR12. However, the KR12:Hp-βCd and KR12 alone alter the zeta potential of cells to a similar extent, suggesting a similar level of membrane interaction. The peptide alone inhibited the proliferation of Caco-2 and A431 cells more efficiently than KR12:Hp-βCd (p < 0.001), but did not show significant cytotoxic effects via the dehydrogenase lactate assay. Both substances were effective in inhibiting the growth of odontopathogenic bacteria, as well as inhibiting Caco-2 epithelial cells. These observations highlight the potential antimicrobial and antiproliferative effects of KR12 peptide alone or associated with Hp-βCd.
local.identifier.orcidhttps://orcid.org/0000-0002-0560-8491
local.identifier.orcidhttps://orcid.org/0000-0001-7656-1849
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://pubs.aip.org/avs/bip/article/11/4/04B307/124601/KR12-peptide-associated-with-cyclodextrin

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