IgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis

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dc.creatorLaura Néspoli Nassar Pansinide Jesus
dc.creatorDaniel Vitor Vasconcelos-santos
dc.creatorJosé Nélio Januário
dc.creatorAndréa Teixeira-carvalho
dc.creatorRicardo Wagner Almeida Vitor
dc.creatorEloísa A.v. Ferro
dc.creatorJosé Roberto Mineo
dc.creatorLilian Maria Garcia Bahia-oliveira
dc.creatorOlindo Assis Martins-filho
dc.creatorElenice Moreira Lemos
dc.creatorAline de Castro Zacche Tonini
dc.creatorGeisa Baptista Barros
dc.creatorJordana Grazziela a. Coelho-dos-reis
dc.creatorSamantha Ribeiro Béla
dc.creatorLis Ribeiro do Valle Antonelli
dc.creatorAnderson Silva Machado
dc.creatorAna Carolina de Aguiar Vasconcelos Carneiro
dc.creatorGláucia Manzan Queiroz Andrade
dc.date.accessioned2023-06-23T20:29:57Z
dc.date.accessioned2025-09-09T00:26:07Z
dc.date.available2023-06-23T20:29:57Z
dc.date.issued2016
dc.format.mimetypepdf
dc.identifier.doi10.1016/j.jim.2015.11.004
dc.identifier.issn00221759
dc.identifier.urihttps://hdl.handle.net/1843/55292
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Immunological Methods
dc.rightsAcesso Restrito
dc.subjectToxoplasma
dc.subjectToxoplasmose Congênita
dc.subjectImunoglobulina A
dc.subjectImunoglobulina G
dc.subject.otherToxoplasma gondii
dc.subject.otherCongenital toxoplasmosis
dc.subject.otherIgA
dc.subject.otherIgG1
dc.titleIgA and IgG1 reactivities assessed by flow cytometry mirror clinical aspects of infants with ocular congenital toxoplasmosis
dc.typeArtigo de periódico
local.citation.epage8
local.citation.spage1
local.citation.volume428
local.description.resumoThis study intended to apply the flow cytometric analysis of IgA and IgG reactivity and intracytoplasmic cytokine analysis to understand and decode the clinical aspects of infants with ocular congenital toxoplasmosis. The Toxoplasma gondii-infected infants (TOXO) were subdivided according to their clinical aspects based on the absence (NRL), presence of active (ARL), active/cicatricial (ACRL) or cicatricial retinochoroidal lesions (CRL) and compared to non-infected controls (NI). The reactivity of anti-T. gondii IgG subclasses resembles the clinical aspects of ocular lesions. IgG and IgG1 discriminate infants with cicatricial lesions (ACRL and CRL) from both ARL and NLR. IgG2 and IgG3 are particularly higher in ACRL and CRL as compared to NLR. No differences were ob served when IgG4 reactivity was evaluated. Thus, the results indicated that the reactivity patterns of IgA, IgG and IgG subclasses are able to discriminate ARL, ACRL and CRL from NLR or NI. IgA and IgG subclasses are relevant serological biomarkers with diagnostic and prognostic applicability, respectively. Moreover, IgA and IgG1 wereclosely related to cytokine production by innate/adaptive immunity cells. IgA reactivity was directly associated to TNF-α-derived from neutrophils, monocytes and CD8+ T-cells, while IgG1 was inversely correlated with IFN-γ-producing CD4+ and CD8+ T-cells but positively correlated with IL-10+ B-cells. These findings provide insights on the relationship between the cytokine production by innate/adaptive immunity and the antibody pattern of infants with ocular congenital toxoplasmosis. In addition, the present study supports the use of flow cytometric serology as a potential tool for the diagnosis and monitoring of ocular lesions in T. gondii-infected infants in the clinical setting.
local.identifier.orcidhttps://orcid.org/0000-0002-0912-3782
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0022175915300600

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