Screening and validating the optimal panel of housekeeping genes for 4t1 breast carcinoma and metastasis studies in mice

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dc.creatorJorge Lucas Nascimento Souza
dc.creatorAna Rafaela Antunes-Porto
dc.creatorIzabela da Silva Oliveira
dc.creatorChiara Cássia Oliveira Amorim
dc.creatorLuiz Octávio Pires
dc.creatorIsabela de Brito Duval
dc.creatorLuisa Vitor Braga do Amaral
dc.creatorFernanda Rezende Souza
dc.creatorEvelyn Ane Oliveira
dc.creatorGeovanni Dantas Cassali
dc.creatorValbert Nascimento Cardoso
dc.creatorSimone Odília Antunes Fernandes
dc.creatorRicardo Toshio Fujiwara
dc.creatorRemo Castro Russo
dc.creatorLilian Lacerda Bueno
dc.date.accessioned2025-08-27T19:24:08Z
dc.date.accessioned2025-09-09T01:02:18Z
dc.date.available2025-08-27T19:24:08Z
dc.date.issued2024-11-02
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doi10.1038/s41598-024-77126-x
dc.identifier.issn20452322
dc.identifier.urihttps://hdl.handle.net/1843/84631
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.rightsAcesso Aberto
dc.subjectRadioisótopos
dc.subject.otherGenes de referência
dc.subject.otherExpressão gênica
dc.subject.otherMetástase experimental
dc.subject.otherRT-qPCR
dc.subject.otherRadioisótopos
dc.titleScreening and validating the optimal panel of housekeeping genes for 4t1 breast carcinoma and metastasis studies in mice
dc.typeArtigo de periódico
local.citation.epage15
local.citation.issue26476
local.citation.spage1
local.citation.volume14
local.description.resumoThe 4T1 model is extensively employed in murine studies to elucidate the mechanisms underlying the carcinogenesis of triple-negative breast cancer. Molecular biology serves as a cornerstone in these investigations. However, accurate gene expression analyses necessitate data normalization employing housekeeping genes (HKGs) to avert spurious results. Here, we initially delve into the characteristics of the tumor evolution induced by 4T1 in mice, underscoring the imperative for additional tools for tumor monitoring and assessment methods for tracking the animals, thereby facilitating prospective studies employing this methodology. Subsequently, leveraging various software platforms, we assessed ten distinct HKGs (GAPDH, 18 S, ACTB, HPRT1, B2M, GUSB, PGK1, CCSER2, SYMPK, ANKRD17) not hitherto evaluated in the 4T1 breast cancer model, across tumors and diverse tissues afflicted by metastasis. Our principal findings underscore GAPDH as the optimal HKG for gene expression analyses in tumors, while HPRT1 emerged as the most stable in the liver and CCSER2 in the lung. These genes demonstrated consistent expression and minimal variation among experimental groups. Furthermore, employing these HKGs for normalization, we assessed TNF-α and VEGF expression in tissues and discerned significant disparities among groups. We posit that this constitutes the inaugural delineation of an ideal HKG for experiments utilizing the 4T1 model, particularly in vivo settings.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://pmc.ncbi.nlm.nih.gov/articles/PMC11531515/

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