Biomarcadores de Lesão Tubular e Glomerular em fetos portadores de Válvula de Uretra Posterior

Abstract

Objective: The posterior urethral valve (PUV) is a congenital anomaly characterized by a membranous structure located in the mucosa of the floor of the prostatic portion of the male posterior urethra. A high percentage of patients with posterior urethral valves develop end-stage renal disease. Previous studies have shown that cytokines, chemokines, and renin-angiotensin system components contribute to renal damage in obstructive uropathies. The present study aimed to measure the levels of urinary biomarkers of fetuses with PUV and compare with the levels of the same molecules in healthy male premature newborns. Methods: Urine samples from 43 fetuses with posterior urethral valve were collected and compared with urine samples from 40 healthy newborns of the same gestational age (controls). Tubular and glomerular biomarker levels were measured by MILLIPLEX® assay kits. The measures molecules were: Osteoprotegerin (OPN), Calbidina Molecule Renal Injury 1 (KIM-1), Protein 1 to fatty acid binding (FABP-1), factor of trefoil 3 (TFF-3), Albumin, Clusterin and Epidermal Growth Factor (EGF). Data were analyzed with the software GraphPad Prism version 6 .0. Results: The PUV Fetuses showed significant reduction in urinary levels of EGF, OPN, Calbidin, Osteoactivin, KIM-1, FABP-1 and TFF-3 compared to controls. Conclusion: Early renal structural and functional impairment influenced the synthesis of glomerular and tubular molecules related to renal physiology in fetuses with PUV.