Cystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury

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dc.creatorPatricia Donado Vaz de Melo
dc.creatorAdriane Pimenta da Costa-Val Bicalho
dc.creatorFabiola de Oliveira Paes-Leme
dc.creatorEliana Matias de Souza
dc.creatorMariah Gois Ceregatti
dc.creatorMarco Túlio Gomes Campos
dc.date.accessioned2023-11-22T22:14:23Z
dc.date.accessioned2025-09-09T00:36:19Z
dc.date.available2023-11-22T22:14:23Z
dc.date.issued2022-06-30
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.format.mimetypepdf
dc.identifier.doiwww.doi.org/10.14202/vetworld.2022.1595-1600
dc.identifier.issn0972-8988
dc.identifier.urihttps://hdl.handle.net/1843/61294
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofVeterinary World
dc.rightsAcesso Aberto
dc.subjectAnimais domésticos
dc.subjectRins
dc.subject.otherCanine
dc.subject.otherImmunoturbidimetry
dc.subject.otherKidney
dc.titleCystatin C assay validation using the immunoturbidimetric method to evaluate the renal function of healthy dogs and dogs with acute renal injury
dc.typeArtigo de periódico
local.citation.epage1600
local.citation.issue6
local.citation.spage1595
local.citation.volume15
local.description.resumoBackground and Aim: Acute kidney injury (AKI) is associated with a grave prognosis. A clinical assessment of kidney function can be performed based on the glomerular filtration rate (GFR). Cystatin C (CysC) can indicate the GFR or kidney function and its measurement is currently performed using immunological methods such as nephelometry, immunoturbidimetry, and enzyme-linked immunosorbent assays in human medicine. However, these techniques are not specific for use in veterinary medicine. This study aimed to validate an immunoturbidimetric assay for serum CysC (sCy) in dogs, determine the sCy reference intervals for healthy dogs, evaluate sCy stability in serum samples, and compare sCy with serum creatinine (sCr) in healthy dogs and dogs with AKI. Materials and Methods: Forty-three dogs were divided into a control group (n = 19) and an AKI group (n = 24). An immunoturbidimetric method including commercially available human CysC calibrated with canine CysC was used to evaluate canine serum samples. Results: An average recovery of 97% was observed for canine serum samples. The reference interval for CysC in healthy dogs was 0.57–1.29 mg/L. The sCy concentration in dogs with AKI was significantly higher (2.82 ± 1.46 mg/L) than in healthy dogs (0.93 ± 0.18 mg/L). Statistical analysis confirmed a strong correlation between sCy and sCr (r = 0.94; p < 0.05) in dogs with AKI. Conclusion: The immunoturbidimetric method of evaluating sCy yielded satisfactory results and can be used for canine samples when a species-specific calibrator is used. Furthermore, sCy is a reliable marker of renal dysfunction in dogs. It is best to store samples for sCy evaluation at temperatures between 4°C and 8°C.
local.identifier.orcidhttps://orcid.org/0000-0002-4013-0147
local.identifier.orcidhttps://orcid.org/0000-0003-3527-7942
local.identifier.orcidhttps://orcid.org/0000-0001-6679-8295
local.identifier.orcidhttps://orcid.org/0000-0001-6247-2255
local.identifier.orcidhttps://orcid.org/0000-0003-0692-6587
local.identifier.orcidhttps://orcid.org/0000-0001-8995-9805
local.publisher.countryBrasil
local.publisher.departmentVET - DEPARTAMENTO DE CLÍNICA E CIRURGIA
local.publisher.departmentVETER - ESCOLA DE VETERINARIA
local.publisher.initialsUFMG
local.url.externahttp://www.veterinaryworld.org/Vol.15/June-2022/25.html

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