Análise das vias metabólicas do ceratocisto odontogênico pré e pós marsupialização e comparação à mucosa oral

Abstract

Odontogenic keratocyst (OKC) is one of the most prevalent odontogenic cysts found in the oral cavity and maxillofacial region and its treatment is associated with high recurrence rates. This lesion presents unique clinical behavior and histopathological characteristics as compared to the other odontogenic cysts. Although there is no consensus on the best treatment modality, marsupialization is a widely used procedure. Studies based on microscopic, immunohistochemical and molecular evaluations have been showing that the OKC epithelium undergoes several modifications after its approximation to the adjacent oral mucosa promoted by marsupialization, showing numerous similarities with the mucosal lining of the oral cavity. On the other hand, the effects of this procedure on the metabolic profile of OKC remain largely unknown. Thus, the present study aims to qualitatively and quantitatively compare the metabolic profile of samples of OKC epithelium before and after marsupialization with matched adjacent oral mucosa biopsies. OKC tissue samples were obtained from patients treated at the Oral and Maxillofacial Surgery facilities of the Faculty of Odontology of UFMG and Odilon Behrens Metropolitan Hospital. Metabolites were extracted from formalin fixed paraffin-embedded tissue samples and an untargeted metabolomic analysis was performed using a HPLC-qTOF. Differential abundance of metabolites and regulation of biological pathways among OKCs before and after marsupialization and matched adjacent oral mucosa were identified by bioinformatics and statistical analyses. Our findings revealed that the metabolic profile of the OKC epithelium that maintained contact with the oral mucosa during marsupialization is more similar to the adjacent oral mucosa than to non-treated lesions.