Miniaturized Spectrophotometric Method for Quantification of Tannins in Pomegranate (Punica granatum L.) Fruit Peel Dried Extracts

dc.creatorJuliana Machado Brêtas
dc.creatorDiego Beltrão Pereira
dc.creatorIsabela da Costa César
dc.creatorGerson Antônio Pianetti
dc.date.accessioned2023-07-17T20:24:44Z
dc.date.accessioned2025-09-09T01:31:56Z
dc.date.available2023-07-17T20:24:44Z
dc.date.issued2020
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttp://doi.org/10.2174/1573411015666181204115035
dc.identifier.issn1573-4110
dc.identifier.urihttps://hdl.handle.net/1843/56500
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCurrent Analytical Chemistry
dc.rightsAcesso Restrito
dc.subjectBrasil
dc.subjectControle de qualidade
dc.subject.otherCommercial dried extracts
dc.subject.otherGreen chemistry
dc.subject.otherMiniaturized spectrophotometric method
dc.subject.otherPomegranate
dc.subject.otherPunica granatum L.
dc.subject.otherQuality control
dc.subject.otherTannis
dc.titleMiniaturized Spectrophotometric Method for Quantification of Tannins in Pomegranate (Punica granatum L.) Fruit Peel Dried Extracts
dc.typeArtigo de periódico
local.citation.epage331
local.citation.issue3
local.citation.spage321
local.citation.volume16
local.description.resumoAbstract: Background : Punica granatum L. (pomegranate) is one of the oldest edible fruits used for the treatment of countless diseases. The bioactivity of this fruit is mainly related to tannins, especially elagitannins. P.granatum fruit peel dried extracts (PPDEs) are widely marketed today and the lack of accessible quality control methods is a key to the variable and questionable quality of these products. Objec tive : To develop a miniaturized spectrophotometric method for quantification of tannins in commercial PPDEs. Me thod : Spectrophotometric method using Folin-Ciocalteu reagent was developed and validated for quantification of total polyphenols and polyphenols not adsorbed by hide powder in 17 samples of commercial PPDEs. Tannins content was calculated by the difference between total polyphenols and polyphenols not adsorbed by hide powder contents and expressed as pyrogallol. The UV/Visible microplate spectrophotometer was operated at 760 nm. Results : The developed miniaturized method was precise, accurate, selective and linear, and has no rotational or translational matrix effect. This method significantly reduced the cost of the analysis: 82.2% with reagents, 84.7% in electricity consumption, and 56.3% in the total analysis time. The wide variability in tannins contents in commercial PPDEs (<1% to 14.20%) shows the importance of quality control, ensuring the therapeutic effect and safety of the extracts. Conc lusion : A miniaturized spectrophotometric method was successfully developed and fully validated, being appropriate for quantification of tannins of PPDEs in quality control routine analysis, as it showed to be a simple and cheaper method.
local.identifier.orcidhttps://orcid.org/0000-0003-1349-9413
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.departmentFARMACIA - FACULDADE DE FARMACIA
local.publisher.initialsUFMG
local.url.externahttps://www.eurekaselect.com/article/94998

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