Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages

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dc.creatorGrazielle Ribeiro Goes
dc.creatorPeter Silva Rocha
dc.creatorAline r. s. Diniz
dc.creatorPedro Henrique Nascimento de Aguiar
dc.creatorCarlos Renato Machado
dc.creatorLeda Quercia Vieira
dc.date.accessioned2023-06-23T22:46:12Z
dc.date.accessioned2025-09-08T23:31:00Z
dc.date.available2023-06-23T22:46:12Z
dc.date.issued2016
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0004555
dc.identifier.issn1935-2735
dc.identifier.urihttps://hdl.handle.net/1843/55303
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPLoS Neglected Tropical Diseases
dc.rightsAcesso Aberto
dc.subjectTrypanosoma cruzi
dc.subjectDoença de chagas
dc.subjectEspécies reativas de nitrogênio
dc.subjectEspécies reativas de oxigênio
dc.subject.otherTrypanosoma cruzi
dc.subject.otherChagas disease
dc.subject.otherespécies reativas de nitrogênio
dc.subject.otherespécies reativas de oxigênio
dc.titleTrypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
dc.title.alternativeTrypanosoma cruzi precisa de um sinal fornecido por espécies reativas de oxigênio para infectar macrófagos
dc.typeArtigo de periódico
local.citation.issue4
local.citation.volume10
local.description.resumoBackground: During Trypanosoma cruzi infection, macrophages produce reactive oxygen species (ROS) in a process called respiratory burst. Several works have aimed to elucidate the role of ROS during T. cruzi infection and the results obtained are sometimes contradictory. T. cruzi has a highly efficiently regulated antioxidant machinery to deal with the oxidative burst, but the parasite macromolecules, particularly DNA, may still suffer oxidative damage. Guanine (G) is the most vulnerable base and its oxidation results in formation of 8-oxoG, a cellular marker of oxidative stress. Methodology/Principal Findings: In order to investigate the contribution of ROS in T. cruzi survival and infection, we utilized mice deficient in the gp91phox (Phox KO) subunit of NADPH oxidase and parasites that overexpress the enzyme EcMutT (from Escherichia coli) or TcMTH (from T. cruzi), which is responsible for removing 8-oxo-dGTP from the nucleotide pool. The modified parasites presented enhanced replication inside murine inflammatory macrophages from C57BL/6 WT mice when compared with control parasites. Interestingly, when Phox KO macrophages were infected with these parasites, we observed a decreased number of all parasites when compared with macrophages from C57BL/6 WT. Scavengers for ROS also decreased parasite growth in WT macrophages. In addition, treatment of macrophages or parasites with hydrogen peroxide increased parasite replication in Phox KO mice and in vivo. Conclusions: Our results indicate a paradoxical role for ROS since modified parasites multiply better inside macrophages, but proliferation is significantly reduced when ROS is removed from the host cell. Our findings suggest that ROS can work like a signaling molecule, contributing to T. cruzi growth inside the cells.
local.identifier.orcidhttps://orcid.org/0000-0002-4942-5021
local.identifier.orcidhttps://orcid.org/0000-0002-8724-3165
local.identifier.orcidhttps://orcid.org/0000-0001-5481-9618
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004555

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