High-carbohydrate diet enhanced the anticontractile effect of perivascular adipose tissue through activation of renin-angiotensin system

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dc.creatorDaniela Esteves Ferreira dos Reis Costa
dc.creatorAna Letícia Malheiros Silveira
dc.creatorGianne Paul Campos
dc.creatorNatália Ribeiro Cabacinha Nóbrega
dc.creatorNatália Ferreira de Araújo
dc.creatorLuciano de Figueiredo Borges
dc.creatorLuciano Dos Santos Aggum Capettini
dc.creatorAdaliene Versiani Matos Ferreira
dc.creatorDaniella Bonaventura
dc.date.accessioned2023-05-25T20:16:41Z
dc.date.accessioned2025-09-09T00:07:50Z
dc.date.available2023-05-25T20:16:41Z
dc.date.issued2021
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3389/fphys.2020.628101
dc.identifier.issn1664-042X
dc.identifier.urihttps://hdl.handle.net/1843/53962
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in physiology
dc.rightsAcesso Aberto
dc.subjectobesidade
dc.subjectSistema Renina-Angiotensina
dc.subjectÓxido Nítrico
dc.subjectPeróxido de Hidrogênio
dc.subject.otherPVAT
dc.subject.otherobesity
dc.subject.otherhigh-carbohydrate diet
dc.subject.otherrenin-angiotensin system
dc.subject.othernitric oxide
dc.subject.otherhydrogen peroxide
dc.titleHigh-carbohydrate diet enhanced the anticontractile effect of perivascular adipose tissue through activation of renin-angiotensin system
dc.typeArtigo de periódico
local.citation.volume11
local.description.resumoThe perivascular adipose tissue (PVAT) is an active endocrine organ responsible for release several substances that influence on vascular tone. Increasing evidence suggest that hyperactivation of the local renin-angiotensin system (RAS) in the PVAT plays a pivotal role in the pathogenesis of cardiometabolic diseases. However, the local RAS contribution to the PVAT control of vascular tone during obesity is still not clear. Since the consumption of a high-carbohydrate diet (HC diet) contributes to obesity inducing a rapid and sustained increase in adiposity, so that the functional activity of PVAT could be modulated, we aimed to evaluate the effect of HC diet on the PVAT control of vascular tone and verify the involvement of RAS in this effect. For that, male Balb/c mice were fed standard or HC diet for 4 weeks. Vascular reactivity, histology, fluorescence, and immunofluorescence analysis were performed in intact thoracic aorta in the presence or absence of PVAT. The results showed that HC diet caused an increase in visceral adiposity and also in the PVAT area. Phenylephrine-induced vasoconstriction was significantly reduced in the HC group only in the presence of PVAT. The anticontractile effect of PVAT induced by HC diet was lost when aortic rings were previously incubated with angiotensin-converting enzyme inhibitor, Mas, and AT2 receptors antagonists, PI3K, nNOS, and iNOS inhibitors, hydrogen peroxide (H2O2) decomposing enzyme or non-selective potassium channels blocker. Immunofluorescence assays showed that both Mas and AT2 receptors as well as nNOS and iNOS isoforms were markedly expressed in the PVAT of the HC group. Furthermore, the PVAT from HC group also exhibited higher nitric oxide (NO) and hydrogen peroxide bioavailability. Taken together, these findings suggest that the anticontractile effect of PVAT induced by HC diet involves the signaling cascade triggered by the renin-angiotensin system through the activation of Mas and AT2 receptors, PI3K, nNOS, and iNOS, leading to increased production of nitric oxide and hydrogen peroxide, and subsequently opening of potassium channels. The contribution of PVAT during HC diet-induced obesity could be a compensatory adaptive characteristic in order to preserve the vascular function.
local.identifier.orcidhttps://orcid.org/0000-0002-0282-5677
local.identifier.orcidhttps://orcid.org/0000-0003-1052-0854
local.identifier.orcidhttps://orcid.org/0000-0002-4524-0695
local.identifier.orcidhttps://orcid.org/0000-0002-6487-9558
local.identifier.orcidhttps://orcid.org/0000-0003-2256-8652
local.publisher.countryBrasil
local.publisher.departmentENF - DEPARTAMENTO DE NUTRIÇÃO
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/articles/10.3389/fphys.2020.628101/full

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