Effectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi)

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dc.creatorFernanda Badotti
dc.creatorFranscislon Silva de Oliveira
dc.creatorCleverson Fernando Garcia
dc.creatorAline Bruna Martins Vaz
dc.creatorPaula Luize Camargos Fonseca
dc.creatorLaila Alves Nahum
dc.creatorGuilherme Oliveira
dc.creatorAristóteles Góes-Neto
dc.date.accessioned2023-06-20T19:26:47Z
dc.date.accessioned2025-09-08T23:57:50Z
dc.date.available2023-06-20T19:26:47Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttp://dx.doi.org/10.1186/s12866-017-0958-x
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/1843/55170
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBMC Microbiology
dc.rightsAcesso Aberto
dc.subjectDNA
dc.subjectCódigo de Barras de DNA Taxonômico
dc.subjectBasidiomycota
dc.subject.otherITS
dc.subject.otherITS1
dc.subject.otherITS2
dc.subject.otherProbable correct identification
dc.subject.otherBarcode gap
dc.subject.otherBasidiomycota
dc.titleEffectiveness of ITS and sub-regions as DNA barcode markers for the identification of Basidiomycota (Fungi)
dc.title.alternativeEficácia de ITS e sub-regiões como marcadores de código de barras de DNA para a identificação de Basidiomycota (Fungos)
dc.typeArtigo de periódico
local.citation.issue42
local.citation.volume17
local.description.resumoBackground: Fungi are among the most abundant and diverse organisms on Earth. However, a substantial amount of the species diversity, relationships, habitats, and life strategies of these microorganisms remain to be discovered and characterized. One important factor hindering progress is the difficulty in correctly identifying fungi. Morphological and molecular characteristics have been applied in such tasks. Later, DNA barcoding has emerged as a new method for the rapid and reliable identification of species. The nrITS region is considered the universal barcode of Fungi, and the ITS1 and ITS2 sub-regions have been applied as metabarcoding markers. In this study, we performed a large-scale analysis of all the available Basidiomycota sequences from GenBank. We carried out a rigorous trimming of the initial dataset based in methodological principals of DNA Barcoding. Two different approaches (PCI and barcode gap) were used to determine the performance of the complete ITS region and sub-regions. Results: For most of the Basidiomycota genera, the three genomic markers performed similarly, i.e., when one was considered a good marker for the identification of a genus, the others were also; the same results were observed when the performance was insufficient. However, based on barcode gap analyses, we identified genomic markers that had a superior identification performance than the others and genomic markers that were not indicated for the identification of some genera. Notably, neither the complete ITS nor the sub-regions were useful in identifying 11 of the 113 Basidiomycota genera. The complex phylogenetic relationships and the presence of cryptic species in some genera are possible explanations of this limitation and are discussed. Conclusions: Knowledge regarding the efficiency and limitations of the barcode markers that are currently used for the identification of organisms is crucial because it benefits research in many areas. Our study provides information that may guide researchers in choosing the most suitable genomic markers for identifying Basidiomycota species.
local.identifier.orcidhttps://orcid.org/0000-0001-9038-4631
local.identifier.orcidhttps://orcid.org/0000-0001-8593-052X
local.identifier.orcidhttps://orcid.org/0000-0001-9354-1401
local.identifier.orcidhttps://orcid.org/0000-0002-3167-6308
local.identifier.orcidhttps://orcid.org/0000-0002-7692-6243
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-0958-x

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