Starch combined with sucrose provokes greater root dentine demineralization than sucrose alone

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dc.creatorSamilly Evangelista Souza
dc.creatorAline Araújo Sampaio
dc.creatorAltair Antoninha del Bel Cury
dc.creatorYuri Wanderley Cavalcanti
dc.creatorAntônio Pedro Ricomini Filho
dc.creatorJaime Aparecido Cury
dc.date.accessioned2023-05-30T13:17:30Z
dc.date.accessioned2025-09-08T22:56:54Z
dc.date.available2023-05-30T13:17:30Z
dc.date.issued2018
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1159/000486427
dc.identifier.issn1421976X
dc.identifier.urihttps://hdl.handle.net/1843/54168
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCaries Research
dc.rightsAcesso Restrito
dc.subjectBiofilms
dc.subjectDemineralization
dc.subjectDentin
dc.subjectStarch
dc.subjectSucrose
dc.subjectBacteria
dc.subjectBiomass
dc.subjectHydrogen-íon concentration
dc.subject.otherBiofilm
dc.subject.otherdemineralization
dc.subject.otherDentine
dc.subject.otherStarch
dc.subject.otherSucrose
dc.titleStarch combined with sucrose provokes greater root dentine demineralization than sucrose alone
dc.typeArtigo de periódico
local.citation.epage330
local.citation.issue4
local.citation.spage323
local.citation.volume52
local.description.resumoSince there is no consensus about whether starch increases the cariogenic potential of sucrose, we used a validated 3-species biofilm model to evaluate if starch combined with sucrose provokes higher root dentine demineralization than sucrose alone. Biofilms (n = 18) composed by Streptococcus mutans (the most cariogenic bacteria), Actinomces naeslundii (which has amylolytic activity), and Streptococcus gordonii (which binds salivary amylase) were formed on root dentine slabs under exposure 8 ×/day to one of the following treatments: 0.9% NaCl, 1% starch, 10% sucrose, or a combination of 1% starch and 10% sucrose. Before each treatment, biofilms were pretreated with human whole saliva for 1 min. The pH of the culture medium was measured daily as an indicator of biofilm acidogenicity. After 96 h of growth, the biofilms were collected, and the biomass, bacteria viability, and polysaccharides were analyzed. Dentine demineralization was assessed by surface hardness loss (% SHL). Biofilm bioarchitecture was analyzed using confocal laser scanning microscopy. Treatment with a starch and sucrose combination provoked higher (p = 0.01) dentine demineralization than sucrose alone (% SHL = 53.2 ± 7.0 vs. 43.2 ± 8.7). This was supported by lower pH values (p = 0.007) of the culture medium after daily exposure to the starch and sucrose combination compared with sucrose (4.89 ± 0.29 vs. 5.19 ± 0.32). Microbiological and biochemical findings did not differ between biofilms treated with the combination of starch and sucrose and sucrose alone (p > 0.05). Our findings give support to the hypothesis that a starch and sucrose combination is more cariogenic for root dentine than sucrose alone
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://karger.com/cre/article/52/4/323/85685/Starch-Combined-with-Sucrose-Provokes-Greater-Root

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