Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice

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dc.creatorAlice Freitas Versiani
dc.creatorAdo Jorio de Vasconcelos
dc.creatorFlávio Guimarães da Fonseca
dc.creatorRuiz Gerhardt Astigarraga
dc.creatorEliseu Soares de Oliveira Rocha
dc.creatorAna Paula Moreira Barboza
dc.creatorErna Geessien Kroon
dc.creatorMilene Alvarenga Rachid
dc.creatorDaniele da Glória de Souza
dc.creatorLuiz Orlando Ladeira
dc.creatorEdel Figueiredo Barbosa Stancioli
dc.date.accessioned2022-06-10T14:52:43Z
dc.date.accessioned2025-09-09T00:34:27Z
dc.date.available2022-06-10T14:52:43Z
dc.date.issued2017-04-04
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1186/s12951-017-0259-4
dc.identifier.issn1477-3155
dc.identifier.urihttps://hdl.handle.net/1843/42419
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Nanobiotechnology
dc.rightsAcesso Aberto
dc.subjectNanotubos de carbono
dc.subjectEspectroscopia de Raman
dc.subjectDengue
dc.subject.otherDengue vaccine
dc.subject.otherCarbon nanotubes
dc.subject.otherSubunit vaccine
dc.subject.otherNanoconjugate
dc.titleMulti-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage1
local.citation.volume15
local.description.resumoBackground: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here.
local.identifier.orcidhttps://orcid.org/0000-0003-1378-5380
local.identifier.orcidhttps://orcid.org/0000-0002-5978-2735
local.identifier.orcidhttps://orcid.org/0000-0002-2854-0768
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
local.publisher.initialsUFMG
local.url.externahttps://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-017-0259-4

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