In vitro developmental competence of adult sheep oocytes treated with roscovitine

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dc.creatorLetícia Ferrari Crocomo
dc.creatorFederica Ariu
dc.creatorLuisa Bogliolo
dc.creatorDaniela Bebbere
dc.creatorSergio Ledda
dc.creatorSony Dimas Bicudo
dc.date.accessioned2023-04-24T21:48:51Z
dc.date.accessioned2025-09-08T23:37:36Z
dc.date.available2023-04-24T21:48:51Z
dc.date.issued2016
dc.identifier.issn0936-6768
dc.identifier.urihttps://hdl.handle.net/1843/52440
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofReproduction in Domestic Animals
dc.rightsAcesso Restrito
dc.subjectOvelha
dc.subjectBiotecnologia
dc.subjectFertilização in vitro
dc.subject.otherOvelha
dc.subject.otherBiotecnologia
dc.subject.otherFertilização in vitro
dc.titleIn vitro developmental competence of adult sheep oocytes treated with roscovitine
dc.typeArtigo de periódico
local.citation.epage281
local.citation.issue2
local.citation.spage276
local.citation.volume51
local.description.resumoThe efficiency of in vitro sheep embryo production is still low compared to that observed in vivo and in other species. In this context, meiotic inhibition strategies emerged as a promising alternative to improve this biotechnology. So, this study aimed to evaluate, for the first time, the effects of roscovitine on in vitro maturation of sheep oocytes and their subsequent embryo development. For this, cumulus-oocyte complexes (COCs) were cultured for 6 h in the presence (Rosco) or absence (Control) of 75 μm roscovitine and, subsequently, in vitro matured (IVM) for 18 h with gonadotropins. At 0 (Immature), 6 and 24 h of culture, the nuclear status of oocytes was evaluated by Hoechst staining. Embryo cleavage and blastocyst formation were recorded 30 h after in vitro fertilization and on day 7 of culture, respectively. Blastocyst quality was evaluated by differential staining. At 6 h, the GV rate in the Rosco treatment (93.8%) was similar to that observed in the Immature oocytes (94.9%) and significantly higher compared to Control (41.3%). After IVM for 18 h, a high and similar proportion of oocytes from Rosco (93.6%) and Control (88.4%) reached the MII stage. In both treatments, approximately 70% of oocytes cleaved and 50% of them developed up to blastocyst. The mean percentage of blastocyst cells, embryoblast, trophoblast and pyknosis did also not differ between Control and Rosco. In conclusion, roscovitine, at the studied experimental conditions, was efficient to reversibly inhibit the meiosis of adult sheep oocytes without detrimental effect on development and quality of the in vitro produced embryos.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://pubmed.ncbi.nlm.nih.gov/26890275/

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