Animal model of arthritis and myositis induced by the Mayaro virus

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dc.creatorFranciele Martins Santos
dc.creatorCyntia Canedo Silva
dc.creatorSergio Oliveira de Paula
dc.creatorRoberto Souza Dias
dc.creatorMichelle Dias Oliveira
dc.creatorIsabella Cristina Toledo Alves Costa
dc.creatorLuciana Souza Fernandes
dc.creatorCarine Ribeiro Pessoa
dc.creatorSérgio Luís Pinto da Matta
dc.creatorVivian Vasconcelos Costa
dc.creatorDanielle da Glória de Souza
dc.date.accessioned2023-07-14T22:16:35Z
dc.date.accessioned2025-09-08T22:51:41Z
dc.date.available2023-07-14T22:16:35Z
dc.date.issued2019
dc.identifier.doihttps://doi.org/10.1371/journal.pntd.0007375
dc.identifier.issn1935-2735
dc.identifier.urihttps://hdl.handle.net/1843/56317
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPLOS Neglected Tropical Diseases
dc.rightsAcesso Aberto
dc.subjectAmérica do Sul
dc.subjectInfecção
dc.subject.otherMayaro virus (MAYV)
dc.subject.otherSouth American
dc.subject.otherClinical manifestations
dc.subject.otherInfection
dc.titleAnimal model of arthritis and myositis induced by the Mayaro virus
dc.typeArtigo de periódico
local.citation.epage20
local.citation.issue5
local.citation.spage1
local.citation.volume13
local.description.resumoBackground The Mayaro virus (MAYV) is an endemic arbovirus in South American countries, where it is responsible for sporadic outbreaks of Mayaro fever. Clinical manifestations include fever, headache, ocular pain, rash, myalgia, and debilitating and persistent polyarthralgia. Understanding the mechanisms associated with MAYV-induced arthritis is of great importance due to the potential for its emergence, urbanization and dispersion to other regions. Methods 15-day old Balb/c mice were infected by two distinct pathways, below the forelimb and in the rear footpad. Animals were observed for a period of 21 days. During this time, they were monitored every 24 hours for disease signs, such as weight loss and muscle weakness. Histological damage in the muscles and joints was evaluated 3, 7, 10, 15 and 20 days post-infection. The cytokine profile in serum and muscles during MAYV infection was evaluated by flow cytometry at different post-infection times. For pain analysis, the animals were submitted to the von Frey test and titre in different organs was evaluated throughout the study to obtain viral kinetics. Findings Infection by two distinct pathways, below the forelimb and in the rear footpad, resulted in a homogeneous viral spread and the development of acute disease in animals. Clinical signs were observed such as ruffled fur, hunched posture, eye irritation and slight gait alteration. In the physical test, both groups presented loss of resistance, which was associated with histopathological damage, including myositis, arthritis, tenosynovitis and periostitis. The immune response was characterized by a strong inflammatory response mediated by the cytokines TNF-α, IL-6 and INF-γ and chemokine MCP-1, followed by the action of IL-10 and IL-4 cytokines. Interpretation The results showed that Balb/c mice represent a promising model to study mechanisms involved in MAYV pathogenesis and for future antiviral testing.
local.identifier.orcidhttps://orcid.org/0000-0002-7102-673X
local.identifier.orcidhttps://orcid.org/0000-0002-0375-8058
local.identifier.orcidhttp://orcid.org/0000-0003-1632-2058
local.identifier.orcidhttp://orcid.org/0000-0003-4327-9454
local.identifier.orcidhttp://orcid.org/0000-0002-3789-5907
local.identifier.orcidhttps://orcid.org/0000-0002-0175-642X
local.identifier.orcidhttps://orcid.org/0000-0003-3432-0769
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007375

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