Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19

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dc.creatorFlávia Fonseca Bagno
dc.creatorSarah Aparecida Rodrigues Sergio
dc.creatorMaria Marta Figueiredo
dc.creatorLara Carvalho Godoi
dc.creatorLuis Adan Flores Andrade
dc.creatorNatália Salazar de Castro
dc.creatorCamila Pereira Soares
dc.creatorAndressa Simões Aguiar
dc.creatorFlávia Jacqueline Almeida
dc.creatorEdimilson Domingos da Silva
dc.creatorAntonio Gomes Pinto Ferreira
dc.creatorEdison Luiz Durigon
dc.creatorRicardo Tostes Gazzinelli
dc.creatorSantuza Maria Ribeiro Teixeira
dc.creatorAna Paula Salles Moura Fernandes
dc.creatorFlávio Guimarães da Fonseca
dc.date.accessioned2025-04-14T15:41:28Z
dc.date.accessioned2025-09-08T23:42:40Z
dc.date.available2025-04-14T15:41:28Z
dc.date.issued2022-08
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.jcvp.2022.100101
dc.identifier.issn2667-0380
dc.identifier.urihttps://hdl.handle.net/1843/81548
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of clinical virology plus
dc.rightsAcesso Aberto
dc.subjectSARS-CoV-2
dc.subjectCOVID-19
dc.subjectDiagnóstico
dc.subjectSorologia
dc.subjectEnsaio de imunoadsorção enzimática
dc.subject.otherSARS-CoV-2
dc.subject.otherCOVID-19
dc.subject.otherDiagnosis
dc.subject.otherSerological assay
dc.subject.otherELISA
dc.subject.otherPrototyping
dc.titleDevelopment and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19
dc.typeArtigo de periódico
local.citation.epage6
local.citation.issue3
local.citation.spage1
local.citation.volume2
local.description.resumoThere is a massive demand to identify alternative methods to detect new cases of COVID-19 as well as to investigate the epidemiology of the disease. In many countries, importation of commercial kits poses a significant impact on their testing capacity and increases the costs for the public health system. We have developed an ELISA to detect IgG antibodies against SARS-CoV-2 using a recombinant viral nucleocapsid (rN) protein expressed in E. coli. Using a total of 894 clinical samples we showed that the rN-ELISA was able to detect IgG antibodies against SARS-CoV-2 with high sensitivity (97.5%) and specificity (96.3%) when compared to a commercial antibody test. After three external validation studies, we showed that the test accuracy was higher than 90%. The rN-ELISA IgG kit constitutes a convenient and specific method for the large-scale determination of SARS-CoV-2 antibodies in human sera with high reliability.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S2667038022000400?via%3Dihub

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