Active chlorine and charcoal affect the in vitro culture of Bambusa vulgaris

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dc.creatorFernanda Cardoso Furlan
dc.creatorNatália Helena Gavilan
dc.creatorAlex Zichner Zorz
dc.creatorEnéas Ricardo Konzen
dc.creatorLeandro Silva de Oliveira
dc.creatorGilvano Ebling Brondani
dc.date.accessioned2023-01-26T21:26:00Z
dc.date.accessioned2025-09-08T22:57:23Z
dc.date.available2023-01-26T21:26:00Z
dc.date.issued2018
dc.identifier.doihttp://dx.doi.org/10.4067/S0717-92002018000100061
dc.identifier.issn0717-9200
dc.identifier.urihttps://hdl.handle.net/1843/49176
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBosque
dc.rightsAcesso Aberto
dc.subjectBambu
dc.subjectAssepsia e antissepsia
dc.subjectContaminação de culturas (Biologia)
dc.subject.otherBamboo
dc.subject.otherAsepsis
dc.subject.otherContamination
dc.subject.otherSprout induction
dc.subject.otherMicropropagation
dc.titleActive chlorine and charcoal affect the in vitro culture of Bambusa vulgaris
dc.typeArtigo de periódico
local.citation.epage70
local.citation.issue1
local.citation.spage61
local.citation.volume39
local.description.resumoWe evaluated the supplementation of the MS culture medium with active chlorine and activated charcoal for the in vitro culture of Bambusa vulgaris. Lateral shoots were collected from field-cultivated plants to obtain explants, which consisted of nodal segments of 1-2 cm length, containing one axillary bud. The MS culture medium was supplemented with the following treatments: E1= 0.004 % v v-1 of active chlorine + 4 g L-1 of charcoal; E2= 0.004 % v v-1 of active chlorine; E3= 4 g L-1 charcoal and E4= control (no active chlorine and no charcoal). The percentage of oxidation, fungal and bacterial contamination, explant establishment, survival, sprout and adventitious rooting were evaluated every 21 d, during 63 d. The use of active chlorine reduced the fungal (28 %) and bacterial (38 %) contamination, being the treatment with the highest percentage of established explants (16 %), survival (68 %) and sprout induction (95 %) after 63 d. After 36 days from the beginning of the multiplication stage, some explants presented spontaneous rooting, without the application of plant growth regulators. An anatomical analysis of sprouts and roots revealed the presence of meristematic zones. Furthermore, it indicated that the emergence of adventitious roots occurred from the nodal region in association with axillary buds. Our results contribute toward the development of more efficient protocols for the in vitro propagation of B. vulgaris, from which we recommend using active chlorine for further assessments.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-92002018000100061&lng=en&nrm=iso

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