Allelic loss in amalgam-associated oral lichenoid lesions compared to oral lichen planus and mucosa

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dc.creatorLeonardo Nogueira Rodrigues
dc.creatorRicardo Santiago Gomez
dc.creatorVanessa de Fátima Bernardes
dc.creatorSílvia Ferreira de Sousa
dc.creatorRaissa Cristina Costa Silva
dc.creatorMauro Henrique Guimarães Abreu
dc.creatorFábio Ramôa Pires
dc.creatorRicardo Alves de Mesquita
dc.creatorLuciana Bastos Rodrigues
dc.creatorLuiz de Marco
dc.creatorCarolina Cavaliéri Gomes
dc.date.accessioned2025-06-02T21:46:30Z
dc.date.accessioned2025-09-09T01:31:37Z
dc.date.available2025-06-02T21:46:30Z
dc.date.issued2017-05
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/odi.12627
dc.identifier.issn1601-0825
dc.identifier.urihttps://hdl.handle.net/1843/82724
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOral Diseases
dc.rightsAcesso Aberto
dc.subjectLoss of heterozygosity
dc.subjectLichen planus, oral
dc.subjectCell proliferation
dc.subjectKi-67 antigen
dc.subjectEvaluation study
dc.subject.otherAmalgam-associated oral lichenoid lesion
dc.subject.otherCell proliferation index
dc.subject.otherLoss of heterozygosity
dc.subject.otherOral lichen planus
dc.titleAllelic loss in amalgam-associated oral lichenoid lesions compared to oral lichen planus and mucosa
dc.typeArtigo de periódico
local.citation.epage476
local.citation.issue4
local.citation.spage471
local.citation.volume23
local.description.resumoBackground: The amalgam-associated oral lichenoid lesion (AAOLL) shows clinical and histopathological features similar to oral lichen planus (OLP). Molecular researches to improve knowledge of pathogenesis and clinical behavior of AAOLL are still scarce. Objective: We investigated for the first time the use of loss of heterozygosity (LOH) as a molecular approach for genetic characterization of AAOLL in comparison with OLP and evaluated the cell proliferation index. Materials and methods: The sample comprised nine AAOLLs, 10 OLPs, and eight NOMs matched by patients' gender and age. LOH was assessed using polymorphic microsatellite markers at chromosomes 9p (D9S157, D9S162, D9S171), 11q (D11S1369), and 17p (TP53, AFM238WF2). Cell proliferation was assessed by immunohistochemical expression of Ki-67 (MIB-1). The association between LOH and Ki-67 was investigated. Results: Loss of heterozygosity occurred in 5/9 AAOLLs and in 2/10 OLPs in at least one marker each, while NOM showed no LOH. Cell proliferation index in AAOLL ranged from 2 to 23%. There was no association between cell proliferation and LOH, independent of the marker. Conclusion: Our study shows that the profile of molecular changes in AAOLL and OLP, evaluated by LOH and Ki-67 expression, is similar. Additional studies including larger samples should be performed to confirm or to refute our findings. Keywords: amalgam-associated oral lichenoid lesion; cell proliferation index; loss of heterozygosity; oral lichen planus.
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0003-0194-7434
local.identifier.orcidhttps://orcid.org/0000-0001-7820-4749
local.identifier.orcidhttps://orcid.org/0000-0001-8794-5725
local.identifier.orcidhttps://orcid.org/0000-0003-0317-8878
local.identifier.orcidhttps://orcid.org/0000-0003-3207-4007
local.identifier.orcidhttps://orcid.org/0000-0002-9053-7201
local.identifier.orcidhttps://orcid.org/0000-0003-2535-7439
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.publisher.countryBrasil
local.publisher.departmentENF - DEPARTAMENTO DE NUTRIÇÃO
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/odi.12627

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