Healing response of rat pulp treated with an injectable keratin hydrogel

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dc.creatorLavany Aajay Sharma
dc.creatorRobert Love
dc.creatorMohammad Ali
dc.creatorAjay Sharma
dc.creatorSoraia Macari
dc.creatorAvadhoot Avadhani
dc.creatorGeorge Dias
dc.date.accessioned2023-03-22T00:40:59Z
dc.date.accessioned2025-09-09T01:33:13Z
dc.date.available2023-03-22T00:40:59Z
dc.date.issued2017-04-20
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.5301/jabfm.5000346
dc.identifier.issn22808000
dc.identifier.urihttps://hdl.handle.net/1843/51111
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Applied Biomaterials & Functional Materials
dc.rightsAcesso Aberto
dc.subjectKeratins
dc.subjectTooth
dc.subjectDental pulp cavity
dc.subjectProteins
dc.subjectBiocompatible materials
dc.subjectDentin
dc.subjectRegenerative medicine
dc.titleHealing response of rat pulp treated with an injectable keratin hydrogel
dc.typeArtigo de periódico
local.citation.epage7
local.citation.spage1
local.citation.volume1
local.description.resumoBackground Keratin has shown promising outcomes as a biomaterial due to its inherent bioactivity, biocompatibility and regenerative effects. The effect of keratin on repair and regeneration of dental tissues has never been studied before. Current therapies to treat pulp tissues involve its replacement with inert, synthetic materials that do not have a proper biological function, leading to failure and tooth loss. This study aimed to develop a biocompatible keratin hydrogel (KH) suitable for pulp therapies. Methods Keratins extracted from sheep wool were isolated, quantified and reconstituted to form KH. Different concentrations of keratin gel suitable for dental application were characterized by rheological analysis. The optimized gel based on flow characteristics was studied further for microstructure including porosity, percentage swelling ratio and contact angle measurements, using analytical tools such as scanning electron microscopy (SEM), micro-computed tomography and goniometer. To assess both biocompatibility and pulpal response, KH was implanted into rat upper molar teeth following partial pulpotomy. After 28 days, the tissue sections were analyzed by histological and immunohistochemical methods to identify dentin matrix protein 1 (DMP-1) formation and compared with control (Ca(OH)2-treated) teeth. Results The results of the study demonstrated a viscous and injectable, porous, dimensionally stable, hydrophilic and biocompatible gel that allowed pulp healing to occur by a reparative response, with widespread DMP-1 expression. Conclusions The findings of this study indicate that keratins can be developed as a biomaterial source for alternate biological treatment options for pulp therapies.
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://journals.sagepub.com/doi/10.5301/jabfm.5000346?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed

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