Survival variability of 12 strains of Bacillus cereus yielded to spray drying of whole milk

Página do item simplificado
dc.creatorVerônica Ortiz Alvarenga
dc.creatorGuilherme Thomaz Pereira Brancini
dc.creatorEric Keven Silva
dc.creatorArthur Kael Rodrigues da Pia
dc.creatorFernanda Bovo Campgnollo
dc.creatorGilberto Úbida Leite Braga
dc.creatorMiriam Dupas Hubinger
dc.creatorAnderson de Souza Sant'Ana
dc.date.accessioned2022-05-16T21:07:13Z
dc.date.accessioned2025-09-09T00:13:07Z
dc.date.available2022-05-16T21:07:13Z
dc.date.issued2018-12
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.identifier.doi10.1016/j.ijfoodmicro.2018.07.020
dc.identifier.issn0168-1605
dc.identifier.urihttps://hdl.handle.net/1843/41726
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofInternational Journal of Food Microbiology
dc.rightsAcesso Restrito
dc.subjectTecnologia de alimentos
dc.subjectLeite
dc.subjectBacillus cereus
dc.subject.otherSpore-forming bacteria
dc.subject.otherThermal processing
dc.subject.otherFoodborne pathogen
dc.subject.otherQuantitative microbiology
dc.subject.otherInactivation parameters
dc.subject.otherHeterogeneity
dc.titleSurvival variability of 12 strains of Bacillus cereus yielded to spray drying of whole milk
dc.typeArtigo de periódico
local.citation.epage89
local.citation.spage80
local.citation.volume286
local.description.resumoThe variability in spore survival during spray drying of 12 Bacillus cereus strains was evaluated. B. cereus spores were inoculated on whole milk (7.2 ± 0.2 log10 spores/g dry weight) and processed in a spray-dryer. Twelve independent experiments were carried out in triplicate. The spore count was determined before and after each drying process, based on the dry weight of whole milk and powdered milk. Then, the number of decimal reductions (γ) caused by the spray drying process was calculated. B. cereus strains presented γ values ranging from 1.0 to 4.7 log10 spores/g dry weight, with a high coefficient of variation (CV) of 46.1%. Cluster analysis allowed to group B. cereus as sensitive (strains 511, 512, 540, 432 and ATCC 14579), intermediate (strains B18, B63, and B86) and resistant strains (strains B3, B94, B51 and 436). Three strains (one of each group) were selected for further investigation and characterization of their physicochemical and molecular (proteomics) differences. Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC) were used to determine physicochemical characteristics and glass transition temperature (Tg), respectively. No differences in signs among the three strains were found in spectra ranging from 900 to 4000 cm−1. The endothermic peak ranged between 54 and 130 °C for strain 540; between 81 and 163 °C for strain B63; and between 110 and 171 °C for strain 436. However, they showed different Tg: 88.82 °C for strain 540; 114.32 °C for strain B63; and 122.70 °C for strain 436. A total of eleven spots were identified by mass spectrometry, with the spore coat protein GerQ, sporulation protein YtfJ (GerW), and peptidyl-prolyl cis-trans isomerase being found in at least two strains. Altogether, the results suggested that the high survival variability of B. cereus spores to the spray drying process seems to be mainly associated with different Tg and protein content. The study highlights the importance of quantifying the effects of this unit operation over the target microorganisms. These data may be relevant for the development of effective measures aiming to control the occurrence of B. cereus in milk powder as well as to reduce spoilage or safety issues associated with the presence of this bacterium in foods, particularly those formulated with milk powder.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0168160518303830?via%3Dihub#!

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG