Composite-derived monomers affect cell viability and cytokine expression in human leukocytes stimulated with porphyromonas gingivalis

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dc.creatorSheyla Omonte Neves
dc.creatorLuísa Mourão Dias Magalhães
dc.creatorJôice Dias Corrêa
dc.creatorWalderez Ornelas Dutra
dc.creatorKenneth John Gollob
dc.creatorTarcília Aparecida da Silva
dc.creatorMartinho Campolina Rebello Horta
dc.creatorPaulo Eduardo Alencar Souza
dc.date.accessioned2025-04-24T21:29:14Z
dc.date.accessioned2025-09-08T23:42:15Z
dc.date.available2025-04-24T21:29:14Z
dc.date.issued2019
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/1678-7757-2018-0529
dc.identifier.issn1678-7765
dc.identifier.urihttps://hdl.handle.net/1843/81833
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of applied oral science
dc.rightsAcesso Aberto
dc.subjectCytokines
dc.subjectPorphyromonas gingivalis
dc.subjectLeukocytes, mononuclear
dc.subjectMaterials testing
dc.subjectComposite resins
dc.subjectApoptosis
dc.subjectBisphenol A-glycidyl methacrylate
dc.subjectInterleukin-1beta
dc.subjectTumor necrosis factor-alpha
dc.subjectBacteria
dc.subjectToxicity
dc.subjectCell survival
dc.subject.otherCytokines
dc.subject.otherPorphyromonas gingivalis
dc.subject.otherMononuclear leukocytes
dc.subject.otherMaterials testing
dc.subject.otherComposite resins
dc.titleComposite-derived monomers affect cell viability and cytokine expression in human leukocytes stimulated with porphyromonas gingivalis
dc.typeArtigo de periódico
local.citation.spagee20180529
local.citation.volume27
local.description.resumoObjectives: Dental composites release unreacted resin monomers into the oral environment, even after polymerization. Periodontal cells are, therefore, exposed to substances that potentially elicit the immune inflammatory response. The underlying molecular mechanisms associated with the interaction between resin monomers and human immune cells found in the gingival crevicular fluid are not fully understood yet. This study investigated the ability of bisphenol A-glycidyl methacrylate (BISGMA), urethane dimethacrylate (UDMA) and triethylene glycol dimethacrylate (TEGDMA) to induce apoptosis and cytokine release by human leukocytes stimulated with a periodontal pathogen. Methodology: Peripheral blood mononuclear cells (PBMC) from 16 healthy individuals were included in this study. To determine the toxicity, the PBMC were incubated for 20 hours, with monomers, for the analysis of cell viability using MTT assay. To evaluate cell death in the populations of monocytes and lymphocytes, they were exposed to sub-lethal doses of each monomer and of heat-inactivated Porphyromonas gingivalis (P. gingivalis) for 5 hours. Secretions of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA after 20 hours. Results: UDMA and TEGDMA induced apoptosis after a short-time exposure. Bacterial challenge induced significant production of IL-1β and TNF-α (p<0.05). TEGDMA reduced the bacterial-induced release of IL-1β and TNF-α, whereas UDMA reduced IL-1β release (p<0.05). These monomers did not affect IL-10 and IL-6 secretion. BISGMA did not significantly interfere in cytokine release. Conclusions: These results show that resin monomers are toxic to PBMC in a dose-dependent manner, and may influence the local immune inflammatory response and tissue damage mechanisms via regulation of bacterial-induced IL-1β and TNF-α secretion by PBMC.
local.identifier.orcidhttps://orcid.org/0000-0001-9957-3295
local.identifier.orcidhttps://orcid.org/0000-0003-0170-6163
local.identifier.orcidhttps://orcid.org/0000-0002-7586-9996
local.identifier.orcidhttps://orcid.org/0000-0003-4184-3867
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0003-0192-5614
local.identifier.orcidhttps://orcid.org/0000-0001-5166-1982
local.identifier.orcidhttps://orcid.org/0000-0003-3924-8356
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/jaos/a/hzhxR4pwGY7ZzCcjzJbWPHk/?format=pdf&lang=en

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