Biocompatibility, induction of mineralisation and antimicrobial activity of experimental pastes based on glass and glass-ceramic materials

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dc.creatorJuliana Maria de Araujo Lopes
dc.creatorFrancine Benetti
dc.creatorGabriely Rezende
dc.creatorMarina Trevelin Souza
dc.creatorLeticia Citelli Conti
dc.creatorEdilson Ervolino
dc.creatorRogério de Castilho Jacinto
dc.creatorEdgar Dutra Zanotto
dc.creatorLuciano Tavares Angelo Cintra
dc.date.accessioned2022-11-25T19:41:21Z
dc.date.accessioned2025-09-09T00:50:23Z
dc.date.available2022-11-25T19:41:21Z
dc.date.issued2020-08-07
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/iej.13382
dc.identifier.issn01432885
dc.identifier.urihttps://hdl.handle.net/1843/47471
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofInternational Endodontic Journal
dc.rightsAcesso Aberto
dc.subjectMaterials testing
dc.subjectBiomineralization
dc.subjectOsteopontin
dc.subjectEnterococcus faecalis
dc.subjectCalcium hydroxide
dc.subjectInflammation
dc.subject.otherBioactive glass
dc.subject.otherBioceramics
dc.subject.otherBiocompatibility
dc.subject.otherBiomineralization
dc.subject.otherOsteopontin
dc.titleBiocompatibility, induction of mineralisation and antimicrobial activity of experimental pastes based on glass and glass-ceramic materials
dc.typeArtigo de periódico
local.citation.epage1505
local.citation.issue11
local.citation.spage1494
local.citation.volume53
local.description.resumoAim To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH)2) paste was used as the positive control. Methodology The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin–eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL−1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal–Wallis and Dunn tests (P < 0.05). Results Most specimens of the control, BS-2P and Ca(OH)2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH)2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH)2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH)2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis. Conclusions Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH)2. Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH)2
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/iej.13382

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