Spiramyin-loaded PLGA implants for the treatment of ocular toxoplasmosis: development, characterization, biocompatibility, and anti-toxoplasma activity

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dc.creatorHarley da Silva Tavares
dc.creatorJessica Ferreira Cardoso
dc.creatorTamires Cunha Almeida
dc.creatorMaria Betânia de Freitas Marques
dc.creatorWagner da Nova Mussel
dc.creatorMariana Campos da Paz Lopes
dc.creatorRodrigo Lambert Oréfice
dc.creatorSilmara Nunes Andrade
dc.creatorFernando de Pilla Varotti
dc.creatorGlenda Nicioli da Silva
dc.creatorGisele Rodrigues da Silva
dc.date.accessioned2026-02-24T16:04:59Z
dc.date.issued2021
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.identifier.doihttps://doi.org/10.1691/ph.2021.0100
dc.identifier.urihttps://hdl.handle.net/1843/1740
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofDie Pharmazie - An International Journal of Pharmaceutical Sciences
dc.rightsAcesso aberto
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectQuímica farmacêutica
dc.subjectToxoplasmose ocular
dc.subjectFourier, Espectroscopia de infravermelho por transformada de
dc.subjectRaios X - Difração
dc.subjectCalorimetria
dc.subjectMicroscopia eletrônica de varredura
dc.subjectCopolímeros
dc.subjectBiofarmacêutica
dc.subject.otherSpiramycin-loaded PLGA implants
dc.subject.otherOcular toxoplasmosis
dc.subject.otherFourier transform infrared spectroscopy
dc.subject.otherX-Ray diffraction
dc.subject.otherDifferential scanning calorimetry
dc.subject.otherScanning electron microscopy
dc.titleSpiramyin-loaded PLGA implants for the treatment of ocular toxoplasmosis: development, characterization, biocompatibility, and anti-toxoplasma activity
dc.typeArtigo de periódico
local.citation.epage76
local.citation.issue2-3
local.citation.spage68
local.citation.volume76
local.creator.Latteshttp://lattes.cnpq.br/8164354651422883
local.creator.Latteshttp://lattes.cnpq.br/0027002746849131
local.creator.Latteshttp://lattes.cnpq.br/7443573533823809
local.creator.Latteshttp://lattes.cnpq.br/8670717808004107
local.creator.Latteshttp://lattes.cnpq.br/3024747722960663
local.creator.Latteshttp://lattes.cnpq.br/0865151742417829
local.creator.Latteshttp://lattes.cnpq.br/4612177644565039
local.creator.Latteshttp://lattes.cnpq.br/6792700863992509
local.creator.Latteshttp://lattes.cnpq.br/8822250034512486
local.creator.Latteshttp://lattes.cnpq.br/3857027043177606
local.creator.Latteshttp://lattes.cnpq.br/3565859229782760
local.description.resumoOcular toxoplasmosis is the major cause of infectious posterior uveitis worldwide, inducing visual field defect and/or blindness. Despite the severity of this disease, an effective treatment is still lacking. In this study, spiramycin-loaded PLGA implants were developed aiming at the treatment of ocular toxoplasmosis. Implants were manufactured by a hot-molding technique, characterized by Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, Differential Scanning Calorimetry, Scanning Electron Microscopy; evaluated in terms of ocular biocompatibility by immunofluorescence, flow cytometry, cell migration, Hen's egg test-chorioallantoic membrane (HET-CAM) irritation test; and investigated in terms of in vitro efficacy against Toxoplasma gondii . Characterization techniques indicated that spiramycin was dispersed into the polymeric chains and both substances preserved their physical structures in implants. The HET-CAM test indicated that implants did not induce hemorrhage or coagulation, being non-irritant to the CAM. ARPE-19 cells showed viability by MTT assay, and normality in cell cycle kinetics and morphology, without stimulating cell death by apoptosis. Finally, they were highly effective against intracellular parasites without inducing human retinal pigment epithelial cell death. In conclusion, spiramycin-loaded PLGA implants represent a promising therapeutic alternative for the local treatment of ocular toxoplasmosis.
local.identifier.orcidhttps://orcid.org/0000-0002-8217-3997
local.identifier.orcidhttps://orcid.org/0000-0001-7236-3853
local.identifier.orcidhttps://orcid.org/0000-0002-5584-3609
local.identifier.orcidhttps://orcid.org/0000-0002-0561-2343
local.identifier.orcidhttps://orcid.org/0000-0002-9768-9830
local.identifier.orcidhttps://orcid.org/0000-0003-2865-7584
local.identifier.orcidhttps://orcid.org/0000-0002-6046-4056
local.identifier.orcidhttps://orcid.org/0000-0002-1975-0827
local.identifier.orcidhttps://orcid.org/0000-0002-2939-7780
local.identifier.orcidhttps://orcid.org/0000-0001-9751-3379
local.identifier.orcidhttps://orcid.org/0000-0001-5920-3521
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA METALÚRGICA
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS DA SAUDE::FARMACIA
local.subject.cnpqCIENCIAS EXATAS E DA TERRA::QUIMICA
local.url.externahttps://www.ingentaconnect.com/contentone/govi/pharmaz/2021/00000076/f0020002/art00004#

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