Perfil de expressão de genes do ciclo celular e de reparo do DNA em ameloblastoma multicístico e unicístico

Abstract

Ameloblastoma is a benign epithelial odontogenic tumor with aggressive behavior and high recurrence rates. The two major variants of ameloblastoma present distinct biological behavior, while multicystic are more aggressive and infiltrative, the unicystic is encapsulate and more indolent. Desregulation in cell cycle gene expression and DNA repair genes are associated with different neoplastic cell characteristics, such as invasion and migration and also can be related to progression of tumors and precancerous lesions. The present study aimed to evaluate and to compare the gene expression profile of multicystic and unicystic ameloblastoma by using quantitative real time PCR array (qPCR array). Cell cycle qPCR array included 84 genes and three samples of each variant were investigated. DNA repair genes qPCR included 48 genes and four samples of each variant were investigated. The results showed fourteen genes differentially expressed in the cell cycle assay, of which ten were upregulated in multicystic compared to unicystic: CCND1, CCNG2, CCNT1, CDK5RAP1, CDK6, HUS1, MAD2L1, MCM5, SKP2, TP53 and four genes showed lower expression in multicystic compared the unicystic: BCCIP, CCNC, CDK1 and MAD2L2. In the DNA repair genes experiment, NTHL1 and CHEK2 showed higher expression in multicystic compared to unicystic. In the evaluated samples, gene transcriptional activity in samples of multicystic variant were higher than unicystic, in both tests. We conclude that relative expression of cell cycle genes and genes related to DNA damage response are differentially expressed in ameloblastoma variants and may be related to the distinct biological behavior. Further studies on the impact of such genes in the biological behavior of ameloblastoma cells might shed light on the mechanisms underlining pathogenesis and evolution of ameloblastoma tumor.