The apical root canal system microbial communities determined by next-generation sequencing

Página do item simplificado
dc.creatorLuciana Carla Nevesde Brito
dc.creatorJanet Doolittle-Hall
dc.creatorChun-Teh Lee
dc.creatorKevin Moss
dc.creatorWilson Bambirra Júnior
dc.creatorWarley Luciano Fonseca Tavares
dc.creatorAntônio Paulino Ribeiro Sobrinho
dc.creatorFlávia Rocha Fonseca Teles
dc.date.accessioned2022-10-21T14:47:29Z
dc.date.accessioned2025-09-08T22:49:38Z
dc.date.available2022-10-21T14:47:29Z
dc.date.issued2020-07-02
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1038/s41598-020-67828-3
dc.identifier.issn20452322
dc.identifier.urihttps://hdl.handle.net/1843/46489
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofScientific Reports
dc.rightsAcesso Aberto
dc.subjectInfections
dc.subjectDental pulp cavity
dc.subjectEndodontics
dc.subjectRNA ribosomal 16s
dc.subjectHost microbial interactions
dc.titleThe apical root canal system microbial communities determined by next-generation sequencing
dc.typeArtigo de periódico
local.citation.epage08
local.citation.issue1
local.citation.spage01
local.citation.volume10
local.description.resumoThe aim of this study was to explore the microbial communities of endodontic infections at their apical portion by 16S rRNA Illumina sequencing and delineate the core microbiome of root canal infections and that of their associated clinical symptomatology. Samples were collected from fifteen subjects presenting one tooth with a root canal infection, and their associated symptoms were recorded. Samples were collected from the apical third of roots using a #10 K file and then amplified using multiple displacement amplification and PCR-amplified with universal primers. Amplicons were sequenced (V3-V4 hypervariable region of the 16S rRNA gene) using MiSeq (Illumina, CA). The microbial composition of the samples was determined using QIIME and HOMINGS. Data were analyzed using t tests and ANOVA. A total of 1,038,656 good quality sequences were obtained, and OTUs were assigned to 10 bacterial phyla, led by Bacteroidetes (51.2%) and Firmicutes (27.1%), and 94 genera were represented primarily by Prevotella (17.9%) and Bacteroidaceae G-1 (14.3%). Symptomatic teeth were associated with higher levels of Porphyromonas (p < 0.05) and Prevotella. P. endodontalis and P. oris were present in both cores. The present study demonstrated the complexity of the root canal microbiome and the "common denominators" of root canal infections and identified taxa whose virulence properties should be further explored. The polymicrobial etiology of endodontic infections has long been established. However, few studies have focused on expanding the breadth and depth of coverage of microbiome-infected root canals at their apical portion
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331743/

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
The apical root canal system microbial communities determined by next generation sequencing.pdf
Tamanho:
2.14 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG