Macrophage migration inhibitory factor drives neutrophil accumulation by facilitating IL-1b production in a murine model of acute gout

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dc.creatorIzabela Galvão
dc.creatorDanielle da Glória de Souza
dc.creatorLin Leng
dc.creatorRichard Bucala
dc.creatorLirândia Pires Sousa
dc.creatorMarcelo Torres Bozza
dc.creatorMauro Martins Teixeira
dc.creatorFlávio Almeida Amaral
dc.creatorMarcelo Torres Bozza
dc.creatorLivia Duarte Tavares
dc.creatorIrla Paula Stopa Rodrigues
dc.creatorCelso Martins Queiroz Junior
dc.creatorVivian Vasconcelos Costa
dc.creatorAlesandra Corte Reis
dc.creatorRene Donizeti Ribeiro Oliveira
dc.creatorPaulo Louzada Junior
dc.date.accessioned2023-07-14T20:34:47Z
dc.date.accessioned2025-09-08T23:27:02Z
dc.date.available2023-07-14T20:34:47Z
dc.date.issued2016
dc.identifier.doihttps://doi.org/10.1189/jlb.3MA0915-418R
dc.identifier.issn1938-3673
dc.identifier.urihttps://hdl.handle.net/1843/56299
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Leukocyte Biology
dc.rightsAcesso Restrito
dc.subjectNeutrofilos
dc.subject.otherMacrophage migration
dc.subject.otherNeutrophil
dc.subject.otherIL-1β production
dc.titleMacrophage migration inhibitory factor drives neutrophil accumulation by facilitating IL-1b production in a murine model of acute gout
dc.typeArtigo de periódico
local.citation.epage1043
local.citation.issue6
local.citation.spage1035
local.citation.volume99
local.description.resumoThis study evaluated the role of macrophage migration inhibitory factor in inflammation caused by monosodium urate crystals. The concentration of macrophage migration inhibitory factor was increased in synovial fluid of patients with acute gout, and there was a positive correlation between intra-articular macrophage migration inhibitory factor and IL-1b concentrations. In mice, the injection of monosodium urate crystals into the knee joint increased the levels of macrophage migration inhibitory factor in macrophages and in inflamed tissue. The injection of recombinant macrophage migration inhibitory factor into the joint of mice reproduced the inflammatory response observed in acute gout, including histologic changes, the recruitment of neutrophils, and increased levels of IL-1b and CXCL1. Importantly, the accumulation of neutrophils and the amount IL-1b in the joints were reduced in macrophage migration inhibitory factor-deficient mice when injected with monosodium urate crystals. We observed a similar effect when we blocked macrophage migration inhibitory factor with (S,R)-3-(4hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid or anti-macrophage migration inhibitory factor. In addition, the blockade of IL-1R and CXCR2 reduced recombinant macrophage migration inhibitory factor-induced neutrophil recruitment. Mechanistically, recombinant macrophage migration inhibitory factor is important for the synthesis of il1b mRNA in vivo and in isolated macrophages. Altogether, macrophage migration inhibitory factor promotes neutrophil accumulation and is important for IL-1b production, which are 2 crucial events contributing to the pathogenesis of acute gout. J. Leukoc. Biol. 99: 1035–1043; 2016.
local.identifier.orcidhttps://orcid.org/0000-0002-9943-8349
local.identifier.orcidhttps://orcid.org/0000-0003-3432-0769
local.identifier.orcidhttps://orcid.org/0000-0002-0605-358X
local.identifier.orcidhttps://orcid.org/0000-0001-5783-5736
local.identifier.orcidhttps://orcid.org/0000-0003-3683-7550
local.identifier.orcidhttps://orcid.org/0000-0002-7309-9238
local.identifier.orcidhttps://orcid.org/0000-0002-4989-1807
local.identifier.orcidhttps://orcid.org/0000-0002-7884-7709
local.identifier.orcidhttps://orcid.org/0000-0002-0175-642X
local.identifier.orcidhttps://orcid.org/0000-0002-8912-5904
local.identifier.orcidhttps://orcid.org/0000-0003-2585-3870
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://jlb.onlinelibrary.wiley.com/doi/full/10.1189/jlb.3MA0915-418R

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