A rapid UPLC method for the simultaneous quantitation of caffeic acid derivatives in dried extracts of Echinacea purpurea

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dc.creatorBárbara Gonçalves de Oliveira
dc.creatorLuiz Filipe Ferreira Santos
dc.creatorGerson Antônio Pianetti
dc.creatorIsabela Costa César
dc.date.accessioned2026-01-21T18:15:29Z
dc.date.issued2021-02-09
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1093/chromsci/bmab001
dc.identifier.issn1945-239X
dc.identifier.urihttps://hdl.handle.net/1843/1458
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Chromatographic Science
dc.rightsAcesso aberto
dc.subjectÁcido cafeico
dc.subject.otherEchinacea purpurea
dc.subject.otherÁcido cafeico
dc.subject.otherCromatografia líquida de ultra-alta eficiência
dc.titleA rapid UPLC method for the simultaneous quantitation of caffeic acid derivatives in dried extracts of Echinacea purpurea
dc.typeArtigo de periódico
local.citation.epage444
local.citation.issue5
local.citation.spage439
local.citation.volume59
local.description.resumoEchinacea purpurea is a traditional medicinal plant widely used as adjuvant for the treatment of respiratory and urinary infections. Caffeic acid derivatives are considered the main active markers, such as chicoric acid, caftaric acid and chlorogenic acid. An analytical method using ultra performance liquid chromatography (UPLC) and diode array detector was developed and validated, to quantify caffeic acid derivatives in commercial dried extracts of EP. UPLC method was developed using a C18 column (50 × 2.1 mm, 1.8 μm), at 30°C. Mobile phase was composed of acetonitrile and 0.05% (v/v) formic acid aqueous solution (10:90), flow rate 0.2 mL/min. Injection volume was 10 μL and detection was performed at 300 and 330 nm. The developed method complied with all required validation parameters, and showed to be linear, precise, accurate, selective and robust for all caffeic acid derivatives. Using the validated method, the levels of caftaric acid (0.110–0.507%w/w), chicoric acid (0.040–0.179%w/w) and chlorogenic acid (0.013–0.084%w/w) were determined in five commercial dried extracts of E. purpurea, with significant variation in the contents between different samples, indicating the need of standardization and control of individual caffeic acid derivatives in commercial extracts.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS DA SAUDE
local.url.externahttps://academic.oup.com/chromsci/article/59/5/439/6131377?login=true

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