The first example of an on-surface enzymatic reaction evaluated by paper spray mass spectrometry (ps-ms): deacetylation of 1,2,3,4-tetra-o-acetyl-β-d-xylopyranose

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dc.creatorJoão Victor Coelho Pimenta
dc.creatorAdão Aparecido Sabino
dc.creatorRodinei Augusti
dc.date.accessioned2023-10-11T20:21:02Z
dc.date.accessioned2025-09-08T23:27:48Z
dc.date.available2023-10-11T20:21:02Z
dc.date.issued2022
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.ijms.2022.116876
dc.identifier.issn1387-3806
dc.identifier.urihttps://hdl.handle.net/1843/59413
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofInternational Journal of Mass Spectrometry
dc.rightsAcesso Aberto
dc.subjectAnálise enzimática
dc.subjectEnzimas - Análise
dc.subjectMacromoléculas
dc.subjectEspectrometria de massa
dc.subjectLipase
dc.subjectRessonancia magnetica nuclear
dc.subjectÁcido clorídrico
dc.subjectCatálise
dc.subject.otherEnzymatic reactionn
dc.subject.otherBCL enzyme
dc.subject.otherMass spectrometry
dc.subject.otherOn-surface reactions
dc.subject.otherPaper spray ionization
dc.subject.otherNuclear magnetic resonance
dc.titleThe first example of an on-surface enzymatic reaction evaluated by paper spray mass spectrometry (ps-ms): deacetylation of 1,2,3,4-tetra-o-acetyl-β-d-xylopyranose
dc.typeArtigo de periódico
local.citation.spage116876
local.citation.volume479
local.description.resumoPaper spray mass spectrometry (PS-MS) is a recent and innovative ambient ionization technique conveniently applied to investigate on-surface chemical reactions. The easy, fast, and efficient PS-MS technique has shown to be a convenient platform to investigate organic reactions, yielding promising results. Lipases are a group of enzymes responsible for esterification and hydrolysis reactions of organic analytes, such as carbohydrates and lipids. Hence, this work describes for the first time an on-surface enzymatic reaction monitored by PS-MS: the deacetylation of 1,2,3,4-tetra-O-acetyl-β-D-xylopyranose (1) induced by the enzyme Burkholderia cepacia lipase (BCL). The reaction was followed over 60 min when the sodium monodeacetylated product (including the respective sodium-bound dimer) and the oxonium intermediate were detected at high intensities. Besides, the influence of BCL concentration and the presence of water and hydrochloric acid were evaluated. Finally, the results described herein demonstrated the feasibility of applying PS-MS to evaluate and get information on on-surface enzymatic reactions, a field still underexplored. These results also open up a new and exciting perspective concerning studying other and perhaps more complex enzymatic reactions in a straightforward manner.
local.identifier.orcidhttps://orcid.org/0000-0003-1265-7694
local.identifier.orcidhttps://orcid.org/0009-0008-2944-5536
local.identifier.orcidhttps://orcid.org/0000-0002-9448-9518
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S1387380622000811

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