Polydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin

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dc.creatorJaqueline de Paula Rezende
dc.creatorGuilherme Max Dias Ferreira
dc.creatorGabriel Max Dias Ferreira
dc.creatorLuis Henrique Mendes da Silva
dc.creatorMaria do Carmo Hespanhol da Silva
dc.creatorMaximiliano Soares Pinto
dc.creatorAna Clarissa dos Santos Pires
dc.date.accessioned2022-03-17T12:50:58Z
dc.date.accessioned2025-09-08T22:55:02Z
dc.date.available2022-03-17T12:50:58Z
dc.date.issued2017-01
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1016/j.msec.2016.09.009
dc.identifier.issn09284931
dc.identifier.urihttps://hdl.handle.net/1843/40170
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMaterials Science and Engineering: C
dc.rightsAcesso Aberto
dc.subjectAlbumina
dc.subjectCopolímeros
dc.subjectLeite - Proteínas
dc.subjectCalorimetria
dc.titlePolydiacetylene/triblock copolymer nanosensor for the detection of native and free bovine serum albumin
dc.typeArtigo de periódico
local.citation.epage543
local.citation.spage535
local.citation.volume70
local.description.resumoBovine serum albumin (BSA) has been recognized as a marker of the cow's health, milk quality, an allergenic protein and as a carrier. Its detection is important in the food, pharmaceutical and medical industries. However, traditional techniques used to detect BSA are often time-consuming, expensive, and show limited sensitivity. This paper describes properties of polydiacetylene-triblock copolymer (L64) nanosensors, synthesized to easily detect BSA. Sensor efficiency was studied as a function of nanosensor composition, polydiacetylene chemical structures, BSA conformation and hydrophobic domain availability, using spectroscopic, calorimetric, light scattering, and electrokinetic analyses. Nanosensors were sensitive to detect the average BSA concentration of milk and dairy products and discriminated between native and denatured protein through naked-eye detectable blue-to-red transition. The standard Gibbs free energy (− 10.44 < ΔG° < − 49.52 kJ M), stoichiometry complex (1 < “n” < 3), and binding constant (6.7 × 102 < Ka < 4.79 × 108 M− 1) of BSA-nanosensor complex formation established a direct relationship between nanosensor response and BSA-nanosensor interaction. BSA-nanosensor interaction was entropically (without cholesterol), and enthalpically driven (with cholesterol). Eugenol-BSA complex did not induce colorimetric transition. Polydiacetylene-L64 nanosensors are potential low-cost sensors for rapid detection of BSA, discriminating between native/denatured and free/bound protein.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0928493116311821?via%3Dihub#!

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