Proof of concept of a novel multiepitope recombinant protein for the serodiagnosis of patients with chagas disease

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dc.creatorJuliana Martins Machado
dc.creatorIsabela Amorim Gonçalves Pereira
dc.creatorAna Clara Gontijo Maia
dc.creatorMariana Ferraz Chaves Francisco
dc.creatorLais Moreira Nogueira
dc.creatorIsadora Braga Gandra
dc.creatorAnna Julia Ribeiro
dc.creatorKamila Alves Silva
dc.creatorCarlos Ananias Aparecido Resende
dc.creatorJonatas Oliveira da Silva
dc.creatorMichelli dos Santos
dc.creatorAna Alice Maia Gonçalves
dc.creatorGrasiele de Sousa Vieira Tavares
dc.creatorMiguel Angel Chávez-Fumagalli
dc.creatorMariana Campos-da-Paz
dc.creatorRodolfo Cordeiro Giunchetti
dc.creatorManoel Otávio da Costa Rocha
dc.creatorAna Thereza Chaves
dc.creatorEduardo Antônio Ferraz Coelho
dc.creatorAlexsandro Sobreira Galdino
dc.date.accessioned2025-02-11T17:51:51Z
dc.date.accessioned2025-09-09T01:01:51Z
dc.date.available2025-02-11T17:51:51Z
dc.date.issued2023-02-14
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/pathogens12020312
dc.identifier.issn2076-0817
dc.identifier.urihttps://hdl.handle.net/1843/79899
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPathogens
dc.rightsAcesso Aberto
dc.subjectDoença de Chagas
dc.subjectProteínas Recombinantes
dc.subjectSaúde pública
dc.subjectDiagnóstico de laboratório
dc.subject.otherMultiepitope
dc.subject.otherRecombinant protein
dc.subject.otherImmunodiagnosis
dc.subject.otherChagas disease
dc.titleProof of concept of a novel multiepitope recombinant protein for the serodiagnosis of patients with chagas disease
dc.typeArtigo de periódico
local.citation.epage11
local.citation.issue2
local.citation.spage1
local.citation.volume12
local.description.resumoChagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome these current diagnosis problems. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. The rTC was constructed based on amino acid sequences from different combinations of Trypanosoma cruzi antigens in the same polypeptide and tested using an enzyme-linked immunosorbent assay (ELISA) to detect different types of Chagas disease. rTC was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, as well as healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These data suggest that rTC has the potential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease.
local.identifier.orcidhttps://orcid.org/0000-0003-1293-8365
local.identifier.orcidhttps://orcid.org/0000-0002-6651-9898
local.identifier.orcidhttps://orcid.org/0000-0003-4240-2035
local.identifier.orcidhttps://orcid.org/0009-0000-4639-6889
local.identifier.orcidhttps://orcid.org/0000-0001-8478-4487
local.identifier.orcidhttps://orcid.org/0000-0003-0785-3084
local.identifier.orcidhttps://orcid.org/0000-0003-0257-6620
local.identifier.orcidhttps://orcid.org/0000-0002-3374-5418
local.identifier.orcidhttps://orcid.org/0000-0001-5055-4915
local.identifier.orcidhttps://orcid.org/0000-0002-8394-4802
local.identifier.orcidhttps://orcid.org/0000-0003-4181-7546
local.identifier.orcidhttps://orcid.org/0000-0002-4721-1767
local.identifier.orcidhttps://orcid.org/0000-0002-6681-9014
local.identifier.orcidhttps://orcid.org/0000-0002-8890-3030
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2076-0817/12/2/312

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