In-depth characterization of antibacterial activity of melittin against Staphylococcus aureus and use in a model of non-surgical MRSA-infected skin wounds

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dc.creatorWilliam Gustavo de Lima
dc.creatorJulio César Moreira de Brito
dc.creatorValbert Nascimento Cardoso
dc.creatorSimone Odília Antunes Fernandes
dc.date.accessioned2022-05-20T19:19:42Z
dc.date.accessioned2025-09-08T23:27:16Z
dc.date.available2022-05-20T19:19:42Z
dc.date.issued2021-01-01
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doi10.1016/j.ejps.2020.105592
dc.identifier.issn0928-0987
dc.identifier.urihttps://hdl.handle.net/1843/41861
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciences
dc.rightsAcesso Restrito
dc.subjectStaphylococcus aureus
dc.subjectMelitina
dc.subjectPotencial antibacteriano
dc.subjectPotencial farmacêutico
dc.subjectPomada
dc.subject.otherMethicillin-resistant Staphylococcus aureus
dc.subject.otherMelittin
dc.subject.otherWound
dc.subject.otherAntimicrobial peptides
dc.titleIn-depth characterization of antibacterial activity of melittin against Staphylococcus aureus and use in a model of non-surgical MRSA-infected skin wounds
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage1
local.citation.volume156
local.description.resumoSkin infections caused by methicillin-resistant Staphylococcus aureus (MRSA) require the development of new and effective topical antibiotics. In this context, melittin, the main component of apitoxin, has a potent antibacterial effect. However, little is known regarding the anti-inflammatory potential this peptide in infection models, or its ability to induce clinically important resistance. Here, we aimed to conduct an in-depth characterization of the antibacterial potential of melittin in vitro and evaluate the pharmaceutical potential of an ointment containing melittin for the treatment of non-surgical infections induced by MRSA. The minimum inhibitory concentration of melittin varied from 0.12 to 4 μM. The antibacterial effect was mainly bactericidal and fast (approximately 0.5 h after incubation) and was maintained even in stationary cells and mature MRSA biofilms. Melittin interacts synergistically with beta-lactams and aminoglycosides, and its ability to form pores in the membrane reverses the resistance of vancomycin-intermediate Staphylococcus aureus (VISA) to amoxicillin, and vancomycin. Its ability to induce resistance in vitro was absent, and melittin was stable in several conditions often associated with infected wounds. In vivo, aointment containing melittin reduced bacterial load and the content of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin-6 (IL-6), and IL-1 beta. Collectively, these data point to melittin as a potential candidate for topical formulations aimed at the treatment of non-surgical infections caused by MRSA.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0928098720303808

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