A simple and sensitive HPLC-FL method for simultaneous determination of angiotensin II receptor antagonists in human plasma

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dc.creatorMateus Araújo Castro e Souza
dc.creatorNaialy Fernandes Araújo Reis
dc.creatorIsabella Marques da Silva Leite
dc.creatorMaria Beatriz de Abreu Glória
dc.creatorGerson Antônio Pianetti
dc.creatorMaria Auxiliadora Parreiras Martins
dc.creatorIsabella Cristina Plácido de Oliveira Pacheco
dc.creatorChristian Fernandes
dc.date.accessioned2022-05-20T21:50:41Z
dc.date.accessioned2025-09-09T01:22:27Z
dc.date.available2022-05-20T21:50:41Z
dc.date.issued2020-09-05
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doi10.1016/j.jpba.2020.113403
dc.identifier.issn0731-7085
dc.identifier.urihttps://hdl.handle.net/1843/41867
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.rightsAcesso Restrito
dc.subjectCromatografia líquida
dc.subjectAnti-hipertensivos
dc.subjectMétodo de fluorescência
dc.subject.otherAngiotensin receptor blockers
dc.subject.otherLiquid chromatography
dc.subject.otherFluorescence
dc.subject.otherProtein precipitation
dc.subject.otherMicroextraction by packed sorbent
dc.subject.otherLiquid-liquid extraction
dc.titleA simple and sensitive HPLC-FL method for simultaneous determination of angiotensin II receptor antagonists in human plasma
dc.typeArtigo de periódico
local.citation.epage8
local.citation.spage1
local.citation.volume188
local.description.resumoAngiotensin II receptor antagonists are one of the most widely used classes of antihypertensive drugs. In this study, an HPLC fluorescence method after protein precipitation (PPT) extraction was developed and validated for determination of olmesartan, losartan, irbesartan, and valsartan in human plasma. The separation was carried out on a Luna cyano (250 × 4.6 mm i.d.; 5 μm particle size) column and the mobile phase was composed of acetonitrile and 0.1 % phosphoric acid in gradient elution, at a flow rate of 1.2 mL min−1. A PPT method was optimized by a two-level factorial design with triplicate at the central point. The parameters that could affect the extraction (sample volume and acetonitrile/plasma volume ratio) were evaluated and the method was compared to microextraction by packed sorbent (MEPS) and liquid-liquid extraction (LLE). The developed method allowed the simultaneous quantification of the analytes employing a simple and cheap sample preparation method and a short chromatographic run (13 min). This method was fully validated showing selectivity, precision, accuracy, and linearity over the range of 25.0–1500.0 ng mL−1 for olmesartan and valsartan, 25.0–2500.0 ng mL−1 for irbesartan, and 35.0–2500.0 ng mL−1 for losartan. Finally, the method was successfully applied in the analysis of human plasma from volunteers.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0731708520312899

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