Occult hepatitis B virus infection in patients with chronic liver disease of different etiology in a Brazilian referral center: comparison of two different hepatitis B virus deoxyribonucleic acid amplification protocols: a cross-sectional study

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dc.creatorAlessandra Coutinho de Faria
dc.creatorBernardo Henrique Mendes Correa
dc.creatorLuciana Costa Faria
dc.creatorPaula Vieira Teixeira Vidigal
dc.creatorMarcelo Antônio Pascoal Xavier
dc.creatorTeresa Cristina Abreu Ferrari
dc.date.accessioned2023-11-06T20:32:04Z
dc.date.accessioned2025-09-08T23:41:45Z
dc.date.available2023-11-06T20:32:04Z
dc.date.issued2022
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/1516-3180.2022.0147.R1.12072022
dc.identifier.issn1806-9460
dc.identifier.urihttps://hdl.handle.net/1843/60512
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofSao Paulo Medical Journal
dc.rightsAcesso Aberto
dc.subjectReação em Cadeia da Polimerase
dc.subjectHepatopatias
dc.subjectHepatite B
dc.subject.otherHepatitis B virus
dc.subject.otherLiver diseases
dc.subject.otherPolymerase chain reaction
dc.titleOccult hepatitis B virus infection in patients with chronic liver disease of different etiology in a Brazilian referral center: comparison of two different hepatitis B virus deoxyribonucleic acid amplification protocols: a cross-sectional study
dc.typeArtigo de periódico
local.citation.epage8
local.citation.issue3
local.citation.spagee2022147
local.citation.volume141
local.description.resumoBACKGROUND: Occult hepatitis B virus infection (OBI) is defined as the presence of hepatitis B virus (HBV) deoxyribonucleic acid (DNA) in the liver of individuals with undetectable hepatitis B virus surface antigen (HBsAg) in the serum. The actual prevalence of OBI and its clinical relevance are not yet fully understood. OBJECTIVE: To evaluate the prevalence of HBV DNA in liver biopsies of HBsAg-negative patients with chronic liver disease of different etiologies in a referral center in Brazil and compare two different HBV DNA amplification protocols to detect HBV. DESIGN AND SETTING: This cross-sectional observational study was conducted at the Liver Outpatient Clinic, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil, between January 2016 and December 2019. METHODS: HBV DNA was investigated in 104 liver biopsy samples from individuals with chronic liver disease of different etiologies, in whom HBsAg was undetectable in serum by nested-polymerase chain reaction (nested-PCR), using two different protocols. RESULTS: OBI, diagnosed by detecting HBV DNA using both protocols, was detected in 6.7% of the 104 individuals investigated. Both protocols showed a good reliability. CONCLUSION: In addition to the differences in the prevalence of HBV infection in different regions, variations in the polymerase chain reaction technique used for HBV DNA amplification may be responsible for the large variations in the prevalence of OBI identified in different studies. There is a need for better standardization of the diagnostic methods used to diagnose this entity.
local.identifier.orcidhttps://orcid.org/0000-0002-7413-8710
local.identifier.orcidhttps://orcid.org/0000-0001-6610-3940
local.identifier.orcidhttps://orcid.org/0000-0002-0226-3491
local.identifier.orcidhttps://orcid.org/0000-0002-7003-5159
local.identifier.orcidhttps://orcid.org/0000-0002-9081-1493
local.identifier.orcidhttps://orcid.org/0000-0001-9459-2294
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.departmentMEDICINA - FACULDADE DE MEDICINA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/spmj/a/Fhmn4SV93wXrBRT4ywWMtPq/?lang=en

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