Mass spectrometry-based proteome profile may be useful to differentiate adenoid cystic carcinoma from polymorphous adenocarcinoma of salivary glands

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dc.creatorFelipe Paiva Fonseca
dc.creatorOslei Paes de Almeida
dc.creatorAlan Roger Santos-Silva
dc.creatorMárcio Ajudarte Lopes
dc.creatorPablo Agustin Vargas
dc.creatorCarolina Carneiro Soares Macedo
dc.creatorSara Ferreira Dos Santos Costa
dc.creatorAdriana Franco Paes Leme
dc.creatorRomênia Ramos Rodrigues
dc.creatorHélder Antônio Rebelo Pontes
dc.creatorAlbina Altemani
dc.creatorWillie F.P. van Heerden
dc.creatorManoela Domingues Martins
dc.date.accessioned2025-08-08T19:03:00Z
dc.date.accessioned2025-09-08T23:16:45Z
dc.date.available2025-08-08T19:03:00Z
dc.date.issued2019-12
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.oooo.2019.07.016
dc.identifier.issn2212-4411
dc.identifier.urihttps://hdl.handle.net/1843/84216
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOral Surgery Oral Medicine oral Pathology Oral Radiology
dc.rightsAcesso Restrito
dc.subjectProteome
dc.subjectProteins
dc.subjectSalivary gland neoplasms
dc.subjectDiagnosis
dc.subjectCarcinoma, adenoid cystic
dc.subjectAdenocarcinoma
dc.titleMass spectrometry-based proteome profile may be useful to differentiate adenoid cystic carcinoma from polymorphous adenocarcinoma of salivary glands
dc.typeArtigo de periódico
local.citation.epage650
local.citation.issue6
local.citation.spage639
local.citation.volume128
local.description.resumoObjective: The aim of this study was to determine the proteome of adenoid cystic carcinoma (AdCC) and polymorphous adenocarcinoma (PAc) and to identify a protein signature useful in distinguishing these two neoplasms. Study design: Ten cases of AdCC and 10 cases of PAc were microdissected for enrichment of neoplastic tissue. The samples were submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the proteomics data were analyzed by using the MaxQuant software. LC-MS/MS spectra were searched against the Human UniProt database, and statistical analyses were performed with Perseus software. Bioinformatic analyses were performed by using discovery-based proteomic data on both tumors. Results: LC-MS/MS analysis identified 1957 proteins. The tumors shared 1590 proteins, and 261 were exclusively identified in AdCC and 106 in PAc. Clustering analysis of the statistically significant proteins clearly separated AdCC from PAc. Protein expression 10 times higher in one group than in the other led to a signature of 16 proteins-6 upregulated in AdCC and 10 in PAc. A new clustering analysis showed reverse regulation and also differentiated both tumors. Conclusions: Global proteomics may be useful in discriminating these two malignant salivary neoplasms that frequently show clinical and microscopic overlaps, but additional validation studies are still necessary to determine the diagnostic potential of the protein signature obtained.
local.identifier.orcidhttps://orcid.org/0000-0003-2494-667X
local.identifier.orcidhttps://orcid.org/0000-0002-6657-4547
local.identifier.orcidhttps://orcid.org/0000-0003-2002-8003
local.identifier.orcidhttps://orcid.org/0000-0003-2040-6617
local.identifier.orcidhttps://orcid.org/0000-0001-6677-0065
local.identifier.orcidhttps://orcid.org/0000-0003-1840-4911
local.identifier.orcidhttps://orcid.org/0000-0002-9858-8206
local.identifier.orcidhttps://orcid.org/0000-0001-5150-9227
local.identifier.orcidhttps://orcid.org/0000-0002-7609-8804
local.identifier.orcidhttps://orcid.org/0000-0002-9944-9734
local.identifier.orcidhttps://orcid.org/0000-0001-8662-5965
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S2212440319313719?via%3Dihub

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