Regulatory monocytes in helminth infections: insights from the modulation during human hookworm infection

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dc.creatorLivia Silva Araújo Passos
dc.creatorLilian Lacerda Bueno
dc.creatorPedro Henrique Gazzinelli-guimarães
dc.creatorTiago Antônio de Oliveira Mendes
dc.creatorAna Clara Gazzinelli Guimarães
dc.creatorDenise da Silveira Lemos
dc.creatorNatasha Delaqua Ricci
dc.creatorRicardo Gonçalves
dc.creatorDaniella Castanheira Bartholomeu
dc.creatorRicardo Toshio Fujiwara
dc.date.accessioned2023-09-14T00:30:11Z
dc.date.accessioned2025-09-09T01:12:48Z
dc.date.available2023-09-14T00:30:11Z
dc.date.issued2017
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1186/s12879-017-2366-0
dc.identifier.issn1471-2334
dc.identifier.urihttps://hdl.handle.net/1843/58651
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBMC Infectious Diseases
dc.rightsAcesso Aberto
dc.subjectMonócitos
dc.subjectInfecções
dc.subject.otherInfecções
dc.subject.otherMonócitos
dc.titleRegulatory monocytes in helminth infections: insights from the modulation during human hookworm infection
dc.typeArtigo de periódico
local.citation.epage9
local.citation.issue1
local.citation.spage1
local.citation.volume17
local.description.resumoBackground While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. Methods Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals. Peripheral blood mononuclear cells (PBMCs) were isolated, and immunophenotyping was conducted by flow cytometry. The expressions of genes encoding human nitric oxide synthase (iNOS), interleukin 4 (IL-4), arginase-1 (Arg-1) and glyceraldehyde 3-phosphate dehydrogenase were quantified by qPCR. Plasma levels of IL-4 were determined by sandwich ELISA. Unpaired t-tests or Mann-Whitney tests were used depending on the data distribution. Results Hookworm infected individuals (HWI) showed a significant increase in the number of monocytes/mm3 (555.2 ± 191.0) compared to that of the non-infected (NI) individuals (120.4 ± 44.7) (p < 0.0001). While the frequencies of CD14+IL-10+ and CD14+IL-12+ cells were significantly reduced in the HWI compared to NI group (p = 0.0289 and p < 0.0001, respectively), the ratio between IL-10/IL-12 producing monocytes was significantly elevated in HWI (p = 0.0004), indicating the potential regulatory activity of these cells. Measurement of IL-4 levels and gene expression of IL-4 and Arg-1 (highly expressed in alternatively activated macrophages) revealed no significant differences between the NI and HWI groups. Interestingly, individuals from the HWI group had higher expression of the iNOS gene (associated with a regulatory profile) (20.27 ± 2.97) compared to the NI group (11.28 ± 1.18, p = 0.0409). Finally, individuals from the HWI group had a significantly higher frequency of CD206+CD23+IL-10+ (7.57 ± 1.96) cells compared to individuals from the NI group (0.35 ± 0.09) (p < 0.001), suggesting that activated monocytes are a potential source of regulatory cytokines during hookworm infection. Conclusions Natural hookworm infection induces a high frequency of circulating monocytes that present a regulatory profile and promote the downmodulation of the proinflammatory response, which may contribute to prolonged survival of the parasite in the host.
local.identifier.orcidhttps://orcid.org/0000-0002-1127-4483
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-017-2366-0#author-information

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