Auxin production by endophytic bacteria isolated from banana trees

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dc.creatorIzabela Cristina Pires Gomes
dc.creatorAmanda Dayane Malta Matos
dc.creatorSilvia Nietsche
dc.creatorAdelica Aparecida Xavier
dc.creatorMárcia Regina Costa
dc.creatorWellington Silva Gomes
dc.creatorMarlon Cristian Toledo Pereira
dc.date.accessioned2022-10-07T15:22:07Z
dc.date.accessioned2025-09-09T00:36:46Z
dc.date.available2022-10-07T15:22:07Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doi10.1590/1678-4324-2017160484
dc.identifier.issn1678-4324
dc.identifier.urihttps://hdl.handle.net/1843/46093
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.rightsAcesso Aberto
dc.subjectPlantas - Efeito da auxina
dc.subjectAlcalóides indólicos
dc.subject.otherAIA
dc.subject.otherBacillus spp.
dc.subject.otherHPLC
dc.subject.othercolorimetria
dc.titleAuxin production by endophytic bacteria isolated from banana trees
dc.typeArtigo de periódico
local.citation.spagee17160484
local.citation.volume60
local.description.resumoThis study aimed to evaluate the capacity for in vitro indole compound synthesis by endophytic bacteria and tocompare the Salkowski colorimetric and high-performance liquid chromatography (HPLC) quantification methods. Initial screenings were performed using 10% tryptic soy agar (TSA) medium with and without L-tryptophan via theSalkowski colorimetric method in a completely randomized design with 40 treatments and three replications. In thesecond experiment, the five most promising isolates were used for the detection and quantification of indoles by HPLC in a completely randomized design with five treatments and three replications. Of the 40 isolates, 16synthesized indole-3-acetic acid (IAA) in the presence of L-tryptophan, and only three synthesized IAA in theabsence of this amino acid. The concentration of IAA determined by absorbance indicated that Agrobacteriumtumefaciens isolate EB.07 showed a higher concentration of IAA in the presence of L-tryptophan (16.61 μ g mL -1 ). HPLC analysis revealed the production of the following four indole compounds by these bacteria: IAA, indole pyruvic acid (IPA), indole lactic acid (ILA), and indole-3-acetaldehyde (IAAld). Significant differences between thetwo estimation methods were observed for two of the five tested isolates: A. tumefaciens (EB.07) and Bacillus pumilus (EB.12). For both isolates, the Salkowski method indicated higher concentrations than those obtained with HPLC. Considering the obtained results, the Salkowski colorimetric method should be used in initial screenings,and HPLC should be used as a complementary method in the selection of isolates with the highest potential tosynthesize indoles.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG

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