Cytotoxicity, inflammation, biomineralization, and immunoexpression of IL-1β and TNF-α promoted by a new bioceramic cement

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dc.creatorLeopoldo Cosme-Silva
dc.creatorAmanda Ferreira Dos Santos
dc.creatorCamila Soares Lopes
dc.creatorRenan Dal Fabbro
dc.creatorFrancine Benetti
dc.creatorJoão Eduardo Gomes-Filho
dc.creatorIndia Olinta de Azevedo Queiroz
dc.creatorEdilson Ervolino
dc.creatorNaiana Viana Viola
dc.date.accessioned2022-11-11T19:31:31Z
dc.date.accessioned2025-09-09T00:31:35Z
dc.date.available2022-11-11T19:31:31Z
dc.date.issued2020
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/1678-7757-2020-0033
dc.identifier.issn16787765
dc.identifier.urihttps://hdl.handle.net/1843/47198
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Applied Oral Science
dc.rightsAcesso Aberto
dc.subjectBiocompatible materials
dc.subjectMaterials testing
dc.subjectPulpotomy
dc.subjectDental pulp cavity
dc.subject.otherBiomaterials
dc.subject.otherCytotoxicity
dc.subject.otherBiocompatibility
dc.subject.otherPulpotomy.
dc.subject.otherDental Pulp
dc.titleCytotoxicity, inflammation, biomineralization, and immunoexpression of IL-1β and TNF-α promoted by a new bioceramic cement
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage01
local.citation.volume28
local.description.resumoAim To evaluate the cytotoxicity, biocompatibility and mineralization capacity of BIO-C PULPO, and MTA. Methodology L929 fibroblasts were cultured and MTT assay was used to determine the material cytotoxicity on 6, 24, and 48 h. A total of 30 male rats (Wistar) aged between 4 and 6 months, weighing between 250 and 300 g were used. Polyethylene tubes containing BIO-C PULPO, MTA, and empty tubes were implanted into dorsal connective tissue. After the experimental periods (7, 15, 30, 60, and 90 days) the tubes were histologically analyzed using hematoxylin-eosin (H&E), immunolabeling of IL-1β and TNF-α, and von Kossa staining, or without staining for polarized light analysis. The average number of inflammatory cells was quantified; the mineralization assessment was determined by the area marked in μm2 and semiquantitative immunolabeling analyses of IL-1β and TNF-α were performed. Then, data underwent statistical analysis with a 5% significance level. Results It was observed that BIO-C PULPO and MTA presented cytocompatibility at 6, 24, and 48 similar or higher than control for all evaluated period. On periods 7 and 15 days, BIO-C PULPO was the material with the highest number of inflammatory cells (p<0.05). On periods 30, 60, and 90 days, BIO-C PULPO and MTA presented similar inflammatory reactions (p>0.05). No statistical differences were found between Control, BIO-C PULPO, and MTA for immunolabeling of IL-1β and TNF-α in the different periods of analysis (p<0.05). Positive von Kossa staining and birefringent structures under polarized light were observed in all analyzed periods in contact with both materials, but larger mineralization area was found with BIO-C PULPO on day 90 (p<0.05). Conclusion BIO-C PULPO was biocompatible and induced mineralization similar to MTA.
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/jaos/a/tq9TvgTL3dspzRgw7NkSDGh/abstract/?lang=en

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