Avaliação da patogenicidade de bactérias de relevância clínica, com fenótipo de resistência a antimicrobianos, em modelos invertebrados alternativos, caenorhabditis elegans e pristionchus pacificus

Abstract

Hospital infections are a worrying factor worldwide due to the high rates of morbidity and mortality. The main causative agents of these substances are bacterial pathogens of great clinical production known by the acronym "ESKAPE" (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.). Among these, the Gram-negative Acinetobacter baumannii MDR (Multidrug-resistant) and Klebsiella pneumoniae (KPC - Klebsiella pneumoniae carbapenemase), are the most prevalent pathogens in the hospital environment, causing a great deal of fatalities. Thus, it is imperative to conduct studies related to aspects of pathogenesis and a microorganism-host interaction. In this context, this study aimed to evaluate the pathogenicity of these microorganisms, with antimicrobial resistance phenotypes, through the analysis of the analysis of invertebrate models, Caenorhabditis elegans and Pristionchus pacificus. The present study revealed that all of them like A. baumannii and K. pneumoniae were pathogenic for the models C. elegans and P. pacificus. The clinical sample of A. baumannii Ab05 was more pathogenic in relation to a reference sample A. baumannii ATCC 19606 and to the clinics Ab06, Ab17, Ab18, Ab21, Ab25 and Ab26 (p <0.05), causing the population to die of C. elegans in 6 days, with LT50 of 4 days. The K. pneumoniae U4, U10, U14, V11 and P5 clinics were the most pathogenic than the reference sample K. pneumoniae ATCC 13883, with an urgency rate in 8 days and LT50 equal to 4, 6, 5, 6 and 5 days, respectively. The U4 sample had a significant difference in relation to ATCC 13883, U10 and V11 (p <0.05). In trials with P. pacificus, clinics Ab06, Ab17 and Ab25 were more pathogenic than a reference sample of A. baumannii ATCC 19606, with the death of P. pacificus in 12 days and LT50 in 10, 10 and 8 days, respectively , however, no difference was observed. Clinics U14, V11 and P5 were more pathogenic than a reference sample ATCC 13883 and U4 and U10 (p <0.05), with the death of P. pacificus in 10 days and LT50 in 8, 8, 10 days, respectively. In short, differences were observed in the susceptibility and survival of these models to these bacterial pathogens with antimicrobial resistance phenotype. In short, differences were observed in the susceptibility and survival of these models to these bacterial pathogens with antimicrobial resistance phenotype.