Eletroextração de aminas biogênicas em leite e vinho e determinação por paper spray-MS e UHPLC-MS/MS

Abstract

Biogenic amines (BA) are widely found in foods and beverages, the consumption of these compounds causes serious undesirable effects on human health. BA are present in many foods such as milk and wine, and a high concentration of these amines can indicate poor quality of raw materials, contamination with microorganisms, improper processing or storage. Unfortunately, the quantification of BA is still problematic due to its low concentration in milk and wine samples and also due to the complexity of the sample with high polarity, lack of chromophores in BA and the presence of potentially interfering substances with similar structures. In the present work, a 3D printing hardware was designed for sample preparation and coupling to the analytical equipment. An electroextraction and analysis method by paper spray ionization mass spectrometry (PS-MS) was optimized for the determination of putrescine, cadaverine, histamine, tryptamine, tyramine, phenylethylamine, agmatine, spermidine and spermine in milk and wine samples. Under the optimized conditions, the method presented adequate selectivity and detectability (in the concentration range of ug/L). In all these applications, the technique proved to be efficient in ionizing the analytes of interest, highlighting its ability to detect substances directly by the developed hardware, low cost compared to conventional techniques, operational simplicity, short analysis time and proving to be advantageous for use in laboratory analytical routines.