Anti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation

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dc.creatorRoger Shoji Sari
dc.creatorAngelo Samir m. Migue
dc.creatorEliane Macedo Sobrinho Santos
dc.creatorAnna Christina de Almeida
dc.creatorAlex Sander Cangussu
dc.creatorEdson Vander Jorge
dc.creatorOtto Domenici Mozzer
dc.creatorHercules Otacilio Santos
dc.creatorWagner Quintilio
dc.creatorIgor Viana Brandi
dc.creatorViviane Aguiar Andrade
dc.date.accessioned2022-06-14T14:12:39Z
dc.date.accessioned2025-09-08T22:50:55Z
dc.date.available2022-06-14T14:12:39Z
dc.date.issued2016
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1016/j.anaerobe.2016.10.013
dc.identifier.issn1075-9964
dc.identifier.urihttps://hdl.handle.net/1843/42502
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAnaerobe
dc.rightsAcesso Aberto
dc.subjectVacina veterinária
dc.subjectQuitosana
dc.subjectAntígenos
dc.subjectAgentes nefrotóxicos
dc.titleAnti-botulism single-shot vaccine using chitosan for protein encapsulation by simple coacervation
dc.typeArtigo de periódico
local.citation.epage187
local.citation.spage182
local.citation.volume42
local.description.resumoThe aim of the present study was to compare the potency and safety of vaccines against Clostridium botulinum (C. botulinum) type C and D formulated with chitosan as controlled release matrix and vaccines formulated in conventional manner using aluminum hydroxide. Parameters were established for the development of chitosan microspheres, using simple coacervation to standardize the use of this polymer in protein encapsulation for vaccine formulation. To formulate a single shot vaccine inactivated antigens of C. botulinum type C and D were used with original toxin titles equal to 5.2 and 6.2 log LD50/ml, respectively. For each antigen a chitosan based solution of 50 mL was prepared. Control vaccines were formulated by mixing toxoid type C and D with aluminum hydroxide [25% Al(OH)3, pH 6.3]. The toxoid sterility, innocuity and potency of vaccines were evaluated as stipulated by MAPA-BRASIL according to ministerial directive no. 23. Encapsulation efficiency of BSA in chitosan was 32.5e40.37%, while that the encapsulation efficiency to toxoid type C was 41,03% (1.94 mg/mL) and of the toxoid type D was 32.30% (1.82 mg/mL). The single shot vaccine formulated using chitosan for protein encapsulation through simple coacervation showed potency and safety similar to conventional vaccine currently used in Brazilian livestock (10 and 2 IU/mL against C. botulinum type C and D, respectively). The present work suggests that our single shot vaccine would be a good option as a cattle vaccine against these C. botulinum type C and D.
local.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117
local.identifier.orcidhttps://orcid.org/0000-0001-6714-7996
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S1075996416301329

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