MicroRNA profiling reveals dysregulated microRNAs and their target gene regulatory networks in cemento-ossifying fibroma

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dc.creatorThaís dos Santos Fontes Pereira
dc.creatorJoão Artur Ricieri Brito
dc.creatorAndré Luiz Sena Guimarães
dc.creatorCarolina Cavaliéri Gomes
dc.creatorJúlio Cesar Tanos de Lacerda
dc.creatorWagner Henriques de Castro
dc.creatorRoney Santos Coimbra
dc.creatorMarina Gonçalves Diniz
dc.creatorRicardo Santiago Gomez
dc.date.accessioned2025-05-26T19:47:25Z
dc.date.accessioned2025-09-08T22:53:41Z
dc.date.available2025-05-26T19:47:25Z
dc.date.issued2018-01
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jop.12650
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/82501
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectBone neoplasms
dc.subjectGene expression
dc.subjectMicroRNAs
dc.subjectOdontogenic tumors
dc.subjectFibroma, ossifying
dc.subjectGenes
dc.subjectSignal transduction
dc.subjectProteoglycans
dc.subjectGene expression regulation
dc.subjectGenetic profile
dc.subjectValidation study
dc.subject.otherBone neoplasms
dc.subject.otherGene expression
dc.subject.othermicroRNA
dc.subject.otherOdontogenic tumor
dc.subject.otherOssifying fibroma
dc.titleMicroRNA profiling reveals dysregulated microRNAs and their target gene regulatory networks in cemento-ossifying fibroma
dc.typeArtigo de periódico
local.citation.epage85
local.citation.issue1
local.citation.spage78
local.citation.volume47
local.description.resumoBackground: Cemento-ossifying fibroma (COF) is a benign fibro-osseous neoplasm of uncertain pathogenesis, and its treatment results in morbidity. MicroRNAs (miRNA) are small non-coding RNAs that regulate gene expression and may represent therapeutic targets. The purpose of the study was to generate a comprehensive miRNA profile of COF compared to normal bone. Additionally, the most relevant pathways and target genes of differentially expressed miRNA were investigated by in silico analysis. Methods: Nine COF and ten normal bone samples were included in the study. miRNA profiling was carried out by using TaqMan® OpenArray® Human microRNA panel containing 754 validated human miRNAs. We identified the most relevant miRNAs target genes through the leader gene approach, using STRING and Cytoscape software. Pathways enrichment analysis was performed using DIANA-miRPath. Results: Eleven miRNAs were downregulated (hsa-miR-95-3p, hsa-miR-141-3p, hsa-miR-205-5p, hsa-miR-223-3p, hsa-miR-31-5p, hsa-miR-944, hsa-miR-200b-3p, hsa-miR-135b-5p, hsa-miR-31-3p, hsa-miR-223-5p and hsa-miR-200c-3p), and five were upregulated (hsa-miR-181a-5p, hsa-miR-181c-5p, hsa-miR-149-5p, hsa-miR-138-5p and hsa-miR-199a-3p) in COF compared to normal bone. Eighteen common target genes were predicted, and the leader genes approach identified the following genes involved in human COF: EZH2, XIAP, MET and TGFBR1. According to the biology of bone and COF, the most relevant KEGG pathways revealed by enrichment analysis were proteoglycans in cancer, miRNAs in cancer, pathways in cancer, p53-, PI3K-Akt-, FoxO- and TGF-beta signalling pathways, which were previously found to be differentially regulated in bone neoplasms, odontogenic tumours and osteogenesis. Conclusion: miRNA dysregulation occurs in COF, and EZH2, XIAP, MET and TGFBR1 are potential targets for functional analysis validation. Keywords: bone neoplasms; gene expression; microRNA; odontogenic tumour; ossifying fibroma.
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.identifier.orcidhttps://orcid.org/0000-0002-5570-3550
local.identifier.orcidhttps://orcid.org/0000-0003-2745-2878
local.identifier.orcidhttps://orcid.org/0000-0002-9204-0573
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://pubmed.ncbi.nlm.nih.gov/29032608/

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