Characterization of progenitor/stem cell population from human dental socket and their multidifferentiation potential

Página do item simplificado
dc.creatorLucyene Miguita Luiz
dc.creatorMaria Cristina Zindel Deboni
dc.creatorPaul Sharpe
dc.creatorAndrea Mantesso Pobocik
dc.date.accessioned2025-09-05T20:10:42Z
dc.date.accessioned2025-09-08T23:37:38Z
dc.date.available2025-09-05T20:10:42Z
dc.date.issued2020-03
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1007/s10561-019-09794-3
dc.identifier.issn1573-6814
dc.identifier.urihttps://hdl.handle.net/1843/84945
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCell and Tissue Banking
dc.rightsAcesso Restrito
dc.subjectCurettage
dc.subjectTooth socket
dc.subjectHumans
dc.subjectMesenchymal stem cells
dc.subjectColony-forming units assay
dc.subjectCD146 antigen
dc.subjectThy-1 antigens
dc.subjectAntibodies, monoclonal
dc.subject.otherAlveolar bone
dc.subject.otherCurettage
dc.subject.otherDental socket
dc.subject.otherHuman
dc.subject.otherMesenchymal
dc.subject.otherStem cell
dc.titleCharacterization of progenitor/stem cell population from human dental socket and their multidifferentiation potential
dc.typeArtigo de periódico
local.citation.epage46
local.citation.issue1
local.citation.spage31
local.citation.volume21
local.description.resumoDental stem cells have many applications in medicine, dentistry and stem cell biology in general due to their easy accessibility and low morbidity. A common surgical manoeuvre after a tooth extraction is the dental socket curettage which is necessary to clean the alveolus and favour alveolar bone healing. This procedure can cause very low morbidity compared to bone marrow collection procedures and the collected material is normally discarded. In order to investigate if the tissue obtained by dental socket curettage after a tooth extraction was a feasible alternative source to isolate human stem cells, we isolated and characterized two different stem cell populations based on STRO-1 and CD146 expression. We were able to collect and grow cells from dental socket of vital and non-vital teeth. Both populations were proliferative, clonogenic and expressed STRO-1, CD146, CD90, NG2, PDGFR-β, which are markers found in stem cells, presented in vitro multiline-differentiation into osteogenic, chondrogenic, and adipogenic tissue, and in vivo transplanted cells formed mineralized tissue. Interestingly, STRO-1+ clonogenic cells presented better multidifferentiation than CD146+ cells. Our results showed that mesenchymal stem cells can be isolated from the tiny tissue collected by dental socket curettage after vital and non-vital tooth extraction and suggest that STRO-1 is an important marker to be used to sort cells with multidifferentiation capacity.
local.identifier.orcidhttps://orcid.org/0000-0002-2604-5210
local.identifier.orcidhttps://orcid.org/0000-0002-2966-6039
local.identifier.orcidhttps://orcid.org/0000-0003-2116-9561
local.identifier.orcidhttps://orcid.org/0000-0002-6640-2884
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s10561-019-09794-3

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG