Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages

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dc.creatorLucilla Silva Oliveira-Mendonça
dc.creatorÉrica Araújo Mendes
dc.creatorJulyanna Oliveira Castro
dc.creatorMylene Melo Silva
dc.creatorAndréa Gonçalves Santos
dc.creatorCarla Martins Kaneto
dc.creatorSandro Oliveira Dias
dc.creatorIvan Bezerra Allaman
dc.creatorMarcos André Vannier-Santos
dc.creatorJuneo Freitas Silva
dc.creatorDanillo Gardenal Augusto
dc.creatorDanielle Oliveira dos Anjos
dc.creatorNailma Aprigio Silva Santos
dc.creatorKamila Pontes Lima
dc.creatorMaria de Fátima Martins Horta
dc.creatorGeorge Rego Albuquerque
dc.creatorMárcio Gilberto Cardoso Costa
dc.creatorIzaltina Silva-Jardim
dc.creatorJane Lima dos Santos
dc.date.accessioned2026-01-06T21:35:37Z
dc.date.issued2022-06-18
dc.identifier.doihttps://doi.org/10.1016/j.crmicr.2022.100145
dc.identifier.issn26665174
dc.identifier.urihttps://hdl.handle.net/1843/1307
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCurrent research in microbial sciences
dc.rightsAcesso aberto
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBiologia molecular
dc.subject.otherBiofungicide
dc.subject.otherTrichoderma
dc.subject.otherTrichoderma microRNAs Autophagy
dc.subject.otherROS
dc.subject.otherInflammasomes
dc.titleTrichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages
dc.typeArtigo de periódico
local.citation.volume3
local.description.resumoTrichoderma spp. are usually considered safe and normally used as biocontrol and biofertilization. Safety for human health is evaluated by several tests that detect various effects such as allergenicity, toxicity, infectivity, and pathogenicity. However, they do not evaluate the effects of the agent upon the immune system. The aim of this study was to investigate the interaction between T. stromaticum spores and mammalian cells to assess the immunomodulatory potential of the spores of this fungus. First, mouse macrophage cell line J774 and human macrophages were exposed to fungal spores and analyzed for structural features, through scanning and transmission electron microscopy. Then, various analysis were performed in human macrophages as to their effect in some functional and molecular aspects of the immune system through immunocytochemistry, flow cytometry and gene expression assays. We demonstrated that T. stromaticum spores induces autophagy and autophagyrelated genes (ATGs) and downmodulate inflammatory mediators, including ROS, NLRP3, the cytokines IL-1β, IL-18, IL-12 and IL-10, as well as TLR2, TLR4, miR-146b and miR-155, which may lead to an augmented susceptibility to pathogens. Our study shows the extension of damages the biofungicide Tricovab® can cause in the innate immune response. Further studies are necessary to elucidate other innate and adaptive immune responses and, consequently, the safety of this fungus when in contact with humans.
local.identifier.orcidhttps://orcid.org/0000-0001-8234-8534
local.identifier.orcidhttps://orcid.org/0000-0001-6874-619X
local.identifier.orcidhttps://orcid.org/0000-0003-4292-153X
local.identifier.orcidhttps://orcid.org/0000-0002-9585-4759
local.identifier.orcidhttps://orcid.org/0000-0003-0883-0466
local.identifier.orcidhttps://orcid.org/0000-0001-9241-2261
local.identifier.orcidhttps://orcid.org/0000-0002-9881-1236
local.identifier.orcidhttps://orcid.org/0000-0001-5665-8547
local.identifier.orcidhttps://orcid.org/0000-0001-8692-1959
local.identifier.orcidhttps://orcid.org/0000-0002-2465-3503
local.identifier.orcidhttps://orcid.org/0000-0002-9009-2716
local.identifier.orcidhttps://orcid.org/0000-0002-0556-8856
local.identifier.orcidhttps://orcid.org/0000-0002-4358-5489
local.identifier.orcidhttps://orcid.org/0000-0001-9247-688X
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS BIOLOGICAS::IMUNOLOGIA
local.url.externahttps://www.sciencedirect.com/science/article/pii/S2666517422000426

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