Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite

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dc.creatorVivianne Carvalho da Cunha Trajano
dc.creatorMaria Esperanza Cortés Segura
dc.creatorRuben Dario Sinisterra
dc.creatorCélia Regina Lanza
dc.creatorCosta
dc.date.accessioned2023-01-25T21:42:10Z
dc.date.accessioned2025-09-08T23:48:12Z
dc.date.available2023-01-25T21:42:10Z
dc.date.issued2016-07
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.msec.2016.03.103
dc.identifier.issn23276053
dc.identifier.urihttps://hdl.handle.net/1843/49151
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Materials Science and Chemical Engineering
dc.rightsAcesso Aberto
dc.subjectDoxycycline
dc.subjectPolymers
dc.subjectCyclodextrins
dc.subjectCompomers
dc.subjectPeriodontics
dc.subjectOsteoblasts
dc.subject.otherb-cyclodextrin
dc.subject.otherdoxycycline
dc.subject.otherPolymers
dc.subject.otherComposite
dc.subject.otherPeriodontal therapy
dc.titleOsteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite
dc.typeArtigo de periódico
local.citation.epage375
local.citation.spage370
local.citation.volume64
local.description.resumoComposites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration. When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites. Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures. The DOX/βCD composite was prepared with a double mixing method. Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days. Composite structures were evaluated by scanning electron microscopy (SEM). Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week. AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days. These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period. SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts. Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite
local.publisher.countryBrasil
local.publisher.departmentFAO - FACULDADE DE ODONTOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0928493116302910?via%3Dihub

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