A rapid single-extraction method for the simultaneous determination of aflatoxins b1, b2, g1, g2, fumonisin b1, and zearalenone in corn meal by ultra performance liquid chromatography tandem mass spectrometry

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dc.creatorFabiano Narciso Paschoal
dc.creatorDaniela de Azevedo Silva
dc.creatorRafael Von Sperling de Souza
dc.creatorMarize Silva de Oliveira
dc.creatorDanilo Augusto Alves Pereira
dc.creatorScheilla Vitorino Carvalho de Souza
dc.date.accessioned2022-05-06T20:55:17Z
dc.date.accessioned2025-09-09T00:16:14Z
dc.date.available2022-05-06T20:55:17Z
dc.date.issued2016
dc.identifier.doi10.1007/s12161-016-0712-2
dc.identifier.issn1936-9751
dc.identifier.urihttps://hdl.handle.net/1843/41438
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFood Analytical Methods
dc.rightsAcesso Restrito
dc.subjectTecnologia de alimentos
dc.subjectFarinha de milho
dc.subjectCromatografia líquida
dc.subjectEspectrometria de massas
dc.subject.otherMycotoxins
dc.subject.otherMass spectrometry
dc.subject.otherFood
dc.subject.otherMultigroup determination
dc.subject.otherSingle-laboratory validation
dc.subject.otherApplication
dc.titleA rapid single-extraction method for the simultaneous determination of aflatoxins b1, b2, g1, g2, fumonisin b1, and zearalenone in corn meal by ultra performance liquid chromatography tandem mass spectrometry
dc.typeArtigo de periódico
local.citation.epage1644
local.citation.spage1631
local.citation.volume10
local.description.resumoA single-extraction method to simultaneously determine aflatoxins (B1, B2, G1, G2), fumonisin B1, and zearalenone in corn meal by ultra performance liquid chromatography tandem mass spectrometry, using a triple quadrupole, was optimized, validated, and applied in an occurrence study. Different extraction solutions were tested, with better performance for methanol/acetonitrile/water (60:20:20, v/v/v). Linearity was observed from 0.25 to 1.50 ng/mL for aflatoxins, from 20 to 120 ng/mL for fumonisin, and from 7.00 to 42.00 ng/mL for zearalenone. Significant matrix effects were shown for all groups. Selectivity was demonstrated, as matrix or spectral interferences were not observed at the predicted retention time window of the target analytes. Average recoveries of 87.57, 93.18, 93.35, 94.20, 78.76, and 95.98% were obtained for aflatoxins (B1, B2, G1, and G2) fumonisin and zearalenone, respectively. A z-score of 0.19 was estimated in a corn certified reference material for fumonisin B1. Maximum relative standard deviation values under repeatability and intermediate precision conditions were determined to be 13.6 and 13.6% for aflatoxins, 3.7 and 6.3% for fumonisin, and 3.5 and 4.0% for zearalenone, respectively. In the occurrence study, 50 samples were analyzed and 44% had measurable levels of fumonisin. Zearalenone was detected in 18%. The proposed method showed considerable advantages, considering environmental impacts, efficiency, and reliability.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s12161-016-0712-2

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