Mensuração das Áreas de Invasão nos Carcinomas em Tumores Mistos da Glândula Mamária Canina e sua Correlação com a Sobrevida Específica

Abstract

Carcinomas in mixed tumors (CMT) are commonly diagnosed in the mammary glands of female dogs, representing 44 % of cases. However, due to the lack of sufficient invasive areas, histological grading is not always viable. This study aimed to measure the areas and quantify the foci of invasion in the stroma of mixed tumor carcinomas and to better understand their biological behavior. Forty-five cases of carcinomas with mixed tumors were evaluated and divided into two groups (with and without metastasis), and the size, perimeter, length, and number of invasive carcinomatous areas were measured. Clinicopathological data were obtained from the requisition forms and the dogs were monitored for survival. Cases diagnosed with carcinoma in mixed tumors with metastasis presented a greater number of foci (p=0.003) and larger invasion areas than cases without metastasis (p=0.006). Furthermore, patients with invasion areas > 3 mm² had shorter survival rates. No relationship was found between the perimeter, length of invasive areas, expression of Ki67, ER, PR, COX-2, and HER-2, and the immunophenotype. It was demonstrated that the number of foci and size of areas of invasion are associated with the status of the regional lymph node, and that histomorphometry is an important tool in veterinary pathology routine, providing valuable information about the biological behavior of carcinomas in mixed tumors.