Characterization of the virulence of three novel clade 2 Clostridioides (Clostridium) difficile strains and a two-year screening in animals and humans in Brazil

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dc.creatorAmanda Nadia Diniz
dc.creatorFrancisco Carlos Faria Lobato
dc.creatorRodrigo Otávio Silveira Silva
dc.creatorLoren Nery Fontoura Moura
dc.creatorDiogo Soares Gonçalves Cruz
dc.creatorCarlos Augusto de Oliveira Junior
dc.creatorHenrique César Pereira Figueiredo
dc.creatorJoão Luís Reis Cunha
dc.creatorEduardo Garcia Vilela
dc.creatorEdward J. Kuijper
dc.creatorMark H Wilcox
dc.date.accessioned2023-12-07T21:25:09Z
dc.date.accessioned2025-09-09T00:37:12Z
dc.date.available2023-12-07T21:25:09Z
dc.date.issued2022-08-26
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0273013
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1843/61840
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPLoS ONE
dc.rightsAcesso Aberto
dc.subjectProgramas de Rastreamento
dc.subjectVirulência
dc.subjectBrasil
dc.subject.otherProgramas de Rastreamento
dc.subject.otherVirulência
dc.subject.otherBrasil
dc.titleCharacterization of the virulence of three novel clade 2 Clostridioides (Clostridium) difficile strains and a two-year screening in animals and humans in Brazil
dc.typeArtigo de periódico
local.citation.epage21
local.citation.issue8
local.citation.spagee0273013
local.citation.volume17
local.description.resumoClostridioides (Clostridium) difficile infection (CDI) is an evolving global healthcare problem, and owing to the diverse and dynamic molecular epidemiology of C. difficile, new strains continue to emerge. In Brazil, only two cases of CDI due to the so called hypervirulent PCR ribotype (RT) 027 belonging to clade 2 have ever been reported, whereas incidence of CDI due to another “hypervirulent” RT078 (clade 5) has not yet been reported. In contrast, novel clade 2 strains have been identified in different hospitals. To better understand the epidemiology of CDIs in Brazil, this study aimed to genotypically and phenotypically characterize three novel Brazilian clade 2 strains (RT883, 884, and 885) isolated from patients with confirmed CDI. In addition, to better understand the circulating RTs, a two-year sampling was conducted in patients from the same hospital and in several domestic and wild animal species. The three strains examined showed lower production of A/B toxins than the control RT027, although two of these strains harbored a truncated tcdC gene. All strains showed swimming motility similar to that of RT027, while RT883 showed higher spore production than the reference strain. In the in vivo hamster model, the lethality of all strains was found to be similar to that of RT027. Both cgMLST and cgMLSA analyses revealed a high genetic similarity among the three-novel clade 2 isolates. In the two-year survey in animals and humans, RT883, 884, and 885 were not detected; however, three new RTs (RT988, RT989, and RT990) were isolated, two of which were genetically related to the three previously reported clade 2 strains. RT106 and RT126 were most frequently detected in humans (47.9%) and animals (57.9%), respectively. Furthermore, RT027 and RT078 were not detected in humans. The results of this study suggest that these novel clade 2 strains have virulence potential and that new strains from clade 2 continue to emerge in our setting, indicating the need for long-term local surveillance.
local.identifier.orcidhttps://orcid.org/0000-0002-5287-5779
local.identifier.orcidhttps://orcid.org/0000-0002-8577-6849
local.identifier.orcidhttps://orcid.org/0000-0002-4565-2868
local.identifier.orcidhttps://orcid.org/0000-0003-0074-2488
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.departmentMEDICINA - FACULDADE DE MEDICINA
local.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0273013

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