Diagnostic evaluation of elisa and chemiluminescent assays as alternative screening tests to indirect immunofluorescence for the detection of antibodies to cellular antigens

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dc.creatorFabianode Almeida Brito
dc.creatorSilvana Maria Elói Santos
dc.creatorGilda Aparecida Ferreira
dc.creatorWilliam Pedrosa
dc.creatorJanaina Gradisse
dc.creatorLara Cristina Costa
dc.creatorSuzane Pretti Figueiredo Neves
dc.date.accessioned2023-06-12T19:41:51Z
dc.date.accessioned2025-09-08T23:44:28Z
dc.date.available2023-06-12T19:41:51Z
dc.date.issued2016-03
dc.format.mimetypepdf
dc.identifier.doi10.1093/ajcp/aqv083
dc.identifier.issn19437722
dc.identifier.urihttps://hdl.handle.net/1843/54828
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAmerican Journal of Clinical Pathology
dc.rightsAcesso Aberto
dc.subjectAnticorpos Antinucleares
dc.subjectAutoanticorpos
dc.subjectLuminescência
dc.subjectTécnicas Imunoenzimáticas
dc.subjectTécnica Indireta de Fluorescência para Anticorpo
dc.subject.otherAntinuclear antibody
dc.subject.otherAutoantibodies
dc.subject.otherSystemic autoimmune rheumatic diseases
dc.subject.otherChemiluminescence
dc.subject.otherEnzyme immunoassay
dc.subject.otherIndirect immunofluorescence
dc.titleDiagnostic evaluation of elisa and chemiluminescent assays as alternative screening tests to indirect immunofluorescence for the detection of antibodies to cellular antigens
dc.typeArtigo de periódico
local.citation.epage331
local.citation.issue3
local.citation.spage323
local.citation.volume145
local.description.resumoObjectives: Detection of antinuclear antibodies (ANAs) plays an important role in the diagnosis of systemic autoim mune rheumatic disease (SARD). Our goal was to evaluate the diagnostic accuracy of three commercially available en zyme-linked immunosorbent assay (ELISA) kits and one chemiluminescent assay for ANA detection, using the clinical diagnostic as the reference standard. Methods: We evaluated serum samples from 143 patients with an established diagnosis of SARD (group 1), 166 patients with infectious diseases and other rheumatic diseases for which the ANA test is not useful in diagnosis (group 2), and 89 outpatients with suspicion of SARD (group 3). Results: The sensitivity for ANA HEp-2, calculated in group 1, was 87.4% and varied between 62.9% and 90.0% for other tests. The specificity for ANA HEp-2, calculated in group 2, was 72.3% and varied between 45.2% and 90.4% for other tests. In group 3, the negative predictive value for ANA Hep-2 was 92.5% and varied between 89.3% and 100% for other tests. Conclusions: Some ELISA kits have comparable or superior diagnostic sensitivity to ANA HEp-2 and could be used as an alternative method for ANA screening, therefore allowing the immediate report of the results with fewer false negatives than ANA HEp-2. Owing to the lower specificity, ELISA-positive samples should be submitted to ANA HEp-2 for confirmation of results.
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE APARELHO LOCOMOTOR
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.departmentMED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTAR
local.publisher.initialsUFMG
local.url.externahttps://academic.oup.com/ajcp/article/145/3/323/1766264?login=true

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