In vitro inhibition of helicobacter pylori and interaction studies of lichen natural products with jack bean urease

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dc.creatorTiago Coelho de Assis Lage
dc.creatorSérgio Antônio Fernandes
dc.creatorLuzia Valentina Modolo
dc.creatorThamilla Maria Silva Maciel
dc.creatorYane Campolina Cachuite Mota
dc.creatorFrancesca Sisto
dc.creatorJosé Ricardo Sabino
dc.creatorJosué Carinhanha Caldas Santos
dc.creatorIsis Martins Figueiredo
dc.creatorCarla Masia
dc.creatorÂngelo de Fátima
dc.date.accessioned2024-07-05T21:13:00Z
dc.date.accessioned2025-09-09T00:19:18Z
dc.date.available2024-07-05T21:13:00Z
dc.date.issued2018
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1039/C8NJ00072G
dc.identifier.issn1144-0546
dc.identifier.urihttps://hdl.handle.net/1843/69774
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofNew Journal of Chemistry
dc.rightsAcesso Restrito
dc.subjectQuímica
dc.subjectBioquímica
dc.subjectHelicobacter pylori
dc.subjectUrease
dc.subjectProdutos naturais
dc.subjectEspectroscopia molecular
dc.subject.otherUrease
dc.subject.otherUrease inhibitors
dc.subject.otherHelicobacter pylori
dc.subject.otherLichen natural products
dc.subject.otherUsnic acid
dc.subject.otherMolecular spectroscopy
dc.titleIn vitro inhibition of helicobacter pylori and interaction studies of lichen natural products with jack bean urease
dc.typeArtigo de periódico
local.citation.epage5366
local.citation.spage5356
local.citation.volume42
local.description.resumoThe interaction of (S)-(−)-usnic acid (2) and fumarprotocetraric acid (3), isolated from Cladonia rappii (lichen), and commercial (R)-(+)-usnic acid (1) with urease was investigated in vitro by molecular spectroscopy at pH 7.4 and kinetics experiments using jack bean type III urease. All lichen compounds tested interact with urease by a statistical quenching mechanism forming non-fluorescent complexes that change the native protein structure. Formation of complexes was spontaneous and stabilized mainly by electrostatic forces, in which the interaction magnitude was determined to be 3 < 2 < 1. Compound 2, whose tridimensional structure is disclosed here, acts as a mixed inhibitor while compounds 1 and 3 function as competitive ones. The (R)-(+)-UA (1) is the most efficient lichen metabolite with respect to impairment of the growth of five H. pylori strains. The minimum inhibitory concentrations (MIC) for the lichen metabolites tested were lower (from 2- to 7.8-fold) than those of omeprazole (reference drug) against all H. pylori strains tested. Overall, the lichen metabolites 1–3 are promising lead compounds for the design of more efficient urease inhibitors for the treatment of H. pylori infections.
local.identifier.orcidhttps://orcid.org/0000-0003-0007-5216
local.identifier.orcidhttps://orcid.org/0000-0003-3054-3316
local.identifier.orcidhttps://orcid.org/0000-0002-8033-0434
local.identifier.orcidhttps://orcid.org/0000-0002-9477-1919
local.identifier.orcidhttps://orcid.org/0000-0002-2853-2215
local.identifier.orcidhttps://orcid.org/0000-0003-0166-2164
local.identifier.orcidhttps://orcid.org/0000-0002-3332-9967
local.identifier.orcidhttps://orcid.org/0000-0002-9525-5123
local.identifier.orcidhttps://orcid.org/0000-0002-1873-5342
local.identifier.orcidhttps://orcid.org/0000-0003-1875-4217
local.identifier.orcidhttps://orcid.org/0000-0003-2344-5590
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://pubs.rsc.org/en/content/articlelanding/2018/nj/c8nj00072g#!

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