Resveratrol acts anti-inflammatory and neuroprotective in an infant rat model of pneumococcal meningitis by modulating the hippocampal mirnome

Página do item simplificado
dc.creatorKarina Barbosa de Queiroz
dc.creatorThaís dos Santos Fontes Pereira
dc.creatorMárcio Sobreira Silva Araújo
dc.creatorRicardo Santiago Gomez
dc.creatorRoney Santos Coimbra
dc.date.accessioned2024-11-19T19:04:36Z
dc.date.accessioned2025-09-08T22:50:36Z
dc.date.available2024-11-19T19:04:36Z
dc.date.issued2018-04-02
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1007/s12035-018-1037-5
dc.identifier.issn1559-1182
dc.identifier.urihttps://hdl.handle.net/1843/78125
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMolecular Neurobiology
dc.rightsAcesso Restrito
dc.subjectMeningitis
dc.subjectNeuroprotection
dc.subjectResveratrol
dc.subjectStreptococcus pneumoniae
dc.subjectGene expression regulation
dc.subjectProteins
dc.subjectApoptosis
dc.subjectMicroRNAs
dc.subject.otherMeningitis
dc.subject.otherNeuroprotection
dc.subject.otherResveratrol
dc.subject.otherStreptococcus pneumoniae
dc.subject.otherMiRNome
dc.subject.otherMicroRNA
dc.titleResveratrol acts anti-inflammatory and neuroprotective in an infant rat model of pneumococcal meningitis by modulating the hippocampal mirnome
dc.typeArtigo de periódico
local.citation.epage8884
local.citation.issue12
local.citation.spage8869
local.citation.volume55
local.description.resumoResveratrol (RSV) is anti-inflammatory and neuroprotective, cross the blood-brain barrier (BBB) and has a safe profile. Besides, RSV modulates the expression of some miRNAs related to neurological disorders. Thus, we hypothesized that RSV can be neuroprotective in pneumococcal meningitis by modulating the global microRNA expression profile (miRNome). Eleven-day old rats were intracysternally infected with S. pneumoniae (~ 2 × 106 c.f.u.) and were orally administered with RSV (50 mg/kg) or vehicle in pre-treatment (before infection) or post-treatment schedules (3 and 18 h p.i.). At 24 h p.i., animals were euthanized and apoptotic cells were counted in the hippocampal dentate gyrus of the right brain hemispheres. The hippocampi from left hemispheres were used for cytokines and chemokines multiplex assay and miRNome profiling with TaqMan OpenArray Rodent MicroRNA. Infected rats treated with RSV had lower apoptotic scores and IL-1β, CCL2, and CCL3 levels when compared to the infected group receiving placebo. Seven miRNAs were down regulated, and 18 were up regulated by pneumococcal acute meningitis. Thirty-seven miRNAs were down regulated, and three were up regulated (hsa-miR-15b-5p, hsa-miR-25-3p, hsa-miR-125b-5p) by the interaction between meningitis and RSV. Pathway enriched analysis revealed that meningitis and RSV modulate the expression of miRNAs targeting critical pathways related to the pathophysiology of bacterial meningitis. Nevertheless, hsa-miR-25-3p and hsa-miR-125b-5p target the transcription factor TEF-1, for which there are binding sites in Il-1β, Ccl2, and Ccl3 genes. RSV is anti-inflammatory and neuroprotective in an infant rat model of pneumococcal meningitis and these positive effects involve the modulation of the hippocampal miRNome.
local.identifier.orcidhttps://orcid.org/0000-0002-7532-3360
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0002-9204-0573
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s12035-018-1037-5

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG